Laboratory of Cancer Research MAKNA-UPM, Institute of Biosciences, University Putra Malaysia, Serdang, 43400 Selangor DE, Malaysia.
Toxicol In Vitro. 2010 Apr;24(3):707-12. doi: 10.1016/j.tiv.2010.01.011. Epub 2010 Feb 1.
The chromosomal aberrations (CA) assay and micronucleus (MN) test were employed to investigate the effect in vitro of zerumbone (ZER) on human chromosomes. ZER is a sesquiterpene compound isolated from the rhizomes of wild ginger, Zingiber zerumbet Smith. The rhizomes of the plant are employed as a traditional medicine for some ailments and as condiments. ZER has been shown to have anti-cancer and apoptosis-inducing properties against various human tumour cells. It has also been shown to be active in vivo against a number of induced malignancies. Studies on ZER genotoxicity in cultured human peripheral blood lymphocytes (PBL) have not been reported so far. Therefore, the present study was undertaken to investigate the ability of ZER to induce chromosomal aberrations and micronuclei formation in human lymphocytes in vitro. Human blood samples were obtained from four healthy, non-smoking males aged 25-35years. Cultures were exposed to the drug for 48h at four final concentrations: 10, 20, 40 and 80 microM. Mitomycin C (MMC) was used as a positive control. The results of chromosomal aberrations assay showed that ZER was not clastogenic, when compared to untreated control, meanwhile MN test results showed a dose-dependent increase in MN formation. The overall clastogenic effect of ZER on human PBL was statistically not significant. In conclusion, ZER is a cytotoxic but not a clastogenic substance in human PBL.
采用染色体畸变（CA）分析和微核（MN）试验，研究了姜烯（ZER）在人染色体体外的作用。ZER 是从野生姜（Zingiber zerumbet Smith）根茎中分离出的一种倍半萜化合物。该植物的根茎被用作一些疾病的传统药物和调味品。ZER 已被证明对各种人类肿瘤细胞具有抗癌和诱导细胞凋亡的特性。它还被证明在体内对多种诱导性恶性肿瘤具有活性。目前为止，尚未有关于 ZER 在培养的人外周血淋巴细胞（PBL）中的遗传毒性的研究报道。因此，本研究旨在探讨 ZER 在体外诱导人淋巴细胞染色体畸变和微核形成的能力。从 4 名年龄在 25-35 岁的健康、不吸烟的男性中获得人血样本。在四个终浓度（10、20、40 和 80 μM）下，将培养物暴露于药物 48 小时。丝裂霉素 C（MMC）用作阳性对照。染色体畸变分析的结果表明，与未经处理的对照组相比，ZER 没有致裂作用，而 MN 试验结果显示 MN 形成呈剂量依赖性增加。ZER 对人 PBL 的整体致裂作用在统计学上不显著。总之，ZER 在人 PBL 中是一种细胞毒性物质，但不是致裂物质。
