School of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 40402, Taiwan.
Evid Based Complement Alternat Med. 2012;2012:406296. doi: 10.1155/2012/406296. Epub 2012 Jul 15.
The present study evaluated the potential genotoxicity of the ethanol extracts from the rhizome of Zingiber zerumbet (L.) Smith (EEZZR) using a standard battery of tests. Chemical analysis with liquid chromatography-tandem mass spectrometry revealed that EEZZR contained Zerumbone (200.3 ± 0.37 μg/g) and 6-gingerol (102.5 ± 0.28 μg/g). There were no increases in the number of revertant colonies with EEZZR at concentrations of 150-5000 μg per plate, regardless of the metabolic activation system (S-9 mix) used in the histidine-dependent auxotrophic mutants of Salmonella typhimurium (strains TA97, TA98, TA100, TA102, and TA1535) compared to the vehicle control. Furthermore, EEZZR at doses of 150-5000 μg mL(-1) did not increase the number of structural aberrations in Chinese hamster lung cells in the presence or absence of S-9 mix. An oral administration of EEZZR to ICR mice, with doses of up to 2000 mg/kg, caused no significant increases in the number of micronucleated polychromatic erythrocytes (MNPCEs) and mean ratio of polychromatic erythrocytes to total erythrocytes. Lastly, RZZEE did not increase the incidence of MNPCEs in bone marrow. Based on these findings, it may be concluded that the use of EEZZR in traditional medicine poses no risk of genotoxicity.
本研究采用标准测试组合评估了姜黄（Zingiber zerumbet（L.）Smith）根茎的乙醇提取物（EEZZR）的潜在遗传毒性。液相色谱-串联质谱化学分析表明，EEZZR 含有莪术烯（200.3 ± 0.37μg/g）和 6-姜酚（102.5 ± 0.28μg/g）。无论使用哪种代谢激活系统（S-9 混合液），在含histidine 的营养缺陷型鼠伤寒沙门氏菌（菌株 TA97、TA98、TA100、TA102 和 TA1535）的依赖组氨酸的变异性辅助突变体中，EEZZR 在浓度为 150-5000μg/平板时，回复菌落数均无增加，与载体对照组相比。此外，EEZZR 在 150-5000μg/mL 剂量下，无论是否存在 S-9 混合液，均未增加中国仓鼠肺细胞的结构异常数。EEZZR 以高达 2000mg/kg 的剂量对 ICR 小鼠进行口服给药，不会导致微核多染红细胞（MNPCE）数量和多染红细胞与总红细胞比值的显著增加。最后，RZZEE 未增加骨髓中 MNPCE 的发生率。基于这些发现，可以得出结论，在传统医学中使用 EEZZR 不会带来遗传毒性的风险。
