Advanced Software Technology & Mechatronics Research Institute of Kyoto, Shimogyo-ku, Kyoto, Japan.
Acta Biomater. 2010 Jul;6(7):2642-9. doi: 10.1016/j.actbio.2010.01.035. Epub 2010 Feb 1.
Surface modification with polyethylene glycol (PEG) has been employed in the development of biomaterials to reduce unfavorable reactions. However, unanticipated body reactions have been reported, with activation of the complement system being suggested as having involvement in these responses. In this study, we prepared a PEG-modified surface on a gold surface using a monolayer of alpha-mercaptoethyl-omega-methoxy-polyoxyethylene. We observed neither protein adsorption nor activation of the complement system on the PEG-modified surface just after preparation. Storage of the PEG-modified surface in a desiccator under ambient light for several days or following ultraviolet irradiation, reflection-adsorption (FTIR-RAS) and X-ray photo spectrometry revealed deterioration of the PEG layer, which became a strong activator of the complement system through the alternative pathway.
表面修饰与聚乙二醇（PEG）已被应用于生物材料的发展，以减少不利的反应。然而，意外的身体反应已被报道，补体系统的激活被认为参与这些反应。在这项研究中，我们准备了一个聚乙二醇修饰的表面上的金表面使用单层的α-巯基乙基-ω-甲氧基-聚氧乙烯。我们既没有观察到蛋白质吸附也没有补体系统的激活在聚乙二醇修饰的表面上，就在准备之后。聚乙二醇修饰的表面在干燥器中储存数天或在紫外线照射后，反射吸附（FTIR-RAS）和 X 射线光电子能谱显示 PEG 层的恶化，这成为补体系统替代途径的强激活剂。
