Ohtsuka A, Piazza A J, Ermak T H, Owen R L
Cell Biology and Aging Section, VA Medical Center, San Francisco, CA.
Cell Tissue Res. 1992 Sep;269(3):403-10. doi: 10.1007/BF00353895.
The distribution patterns of extracellular matrix elements were determined to ascertain whether they play a role in the localization of lymphocytes in discrete T-cell, B-cell and dome antigen-processing domains within Peyer's patches. Antibodies against collagen types I, III and IV, laminin and fibronectin were applied to cryosections of mouse Peyer's patches and localized by direct or indirect immunoperoxidase methods. T-cell domains were identified with a monoclonal antibody against Thy-1.2. Labeled reticular fibers in distinctive patterns were more numerous in parafollicular and dome areas than within follicles. Germinal centers contained few such fibers. In parafollicular areas, fibers were oriented predominantly toward follicle domes; their distribution corresponded to T-cell zones and lymphocyte traffic areas, with their orientation being parallel to the migration pathways of lymphocytes from high endothelial venules to the antigen-processing domes. Subepithelial and subendothelial basal laminae were immunopositive for type-IV collagen, laminin and fibronectin. The dome subepithelial basal lamina had pore-like discontinuities through which lymphocytes migrated to and from the epithelium. The correspondence of the distribution patterns of extracellular matrix to specific functional domains of Peyer's patches suggests that this matrix provides a structural framework for lymphocyte migration and localization.
确定细胞外基质成分的分布模式，以确定它们是否在派尔集合淋巴结内离散的T细胞、B细胞和圆顶状抗原处理区域中淋巴细胞的定位中发挥作用。将针对I型、III型和IV型胶原蛋白、层粘连蛋白和纤连蛋白的抗体应用于小鼠派尔集合淋巴结的冰冻切片，并通过直接或间接免疫过氧化物酶方法进行定位。用抗Thy-1.2单克隆抗体识别T细胞区域。在滤泡旁和圆顶区域，具有独特模式的标记网状纤维比滤泡内更多。生发中心含有很少这样的纤维。在滤泡旁区域，纤维主要朝向滤泡圆顶定向；它们的分布与T细胞区和淋巴细胞运输区域相对应，其定向与淋巴细胞从高内皮微静脉迁移到抗原处理圆顶的迁移途径平行。上皮下和内皮下单层基底膜对IV型胶原蛋白、层粘连蛋白和纤连蛋白呈免疫阳性。圆顶状上皮下基底膜有孔状间断，淋巴细胞通过这些间断进出上皮。细胞外基质的分布模式与派尔集合淋巴结特定功能区域的对应关系表明，这种基质为淋巴细胞迁移和定位提供了结构框架。
