Narayanan S
Department of Pathology, New York Medical College-Metropolitan Hospital Center, NY 10029.
Ann Clin Lab Sci. 1991 Jan-Feb;21(1):12-8.
A perspective on serum alkaline phosphatase isoenzymes in liver disease is provided with a brief discussion of the location of the enzyme in liver and its presumed function. Mechanisms of entry of alkaline phosphatase into serum in liver disease are discussed. Characterization of high molecular weight alkaline phosphatase in obstructive jaundice is reviewed. The relationship between blood group O and the appearance of the intestinal enzyme in sera of such subjects with cirrhosis of liver is discussed. Properties of hepatoma alkaline phosphatase and the genesis of liver alkaline phosphatase in diseases not related to the liver are explored. Methods for detection of serum alkaline phosphatase isoenzymes in liver disease are discussed from the standpoint of the limitations of electrophoretic procedures, and the promise of procedures such as isoelectric focusing and high performance liquid chromatography that are currently non-routine.
本文提供了关于肝病中血清碱性磷酸酶同工酶的观点，并简要讨论了该酶在肝脏中的位置及其假定功能。探讨了肝病时碱性磷酸酶进入血清的机制。综述了梗阻性黄疸中高分子量碱性磷酸酶的特征。讨论了O血型与肝硬化患者血清中出现肠道酶之间的关系。探索了肝癌碱性磷酸酶的特性以及非肝脏相关疾病中肝脏碱性磷酸酶的起源。从电泳方法的局限性以及等电聚焦和高效液相色谱等目前非常规方法的前景角度，讨论了肝病中血清碱性磷酸酶同工酶的检测方法。
