Picarelli Antonio, Libanori Valerio, De Nitto Daniela, Saponara Annarita, Di Tola Marco, Donato Giuseppe
Ann Clin Lab Sci. 2010 Winter;40(1):85-7.
Anti-endomysial and anti-transglutaminase antibodies can be produced in vitro by the intestinal mucosa of celiac disease (CD) patients in clinical remission, when the culture is performed in the presence of gliadin peptides. Our aim was to use this organ culture system as a means to detect the pathognomonic antibodies of celiac disease (CD) in the culture supernatants. Organ culture was performed in the presence of three different activators to evaluate which one induced the strongest antibody response in intestinal mucosa from patients in clinical remission of CD. Our data confirm the high efficiency of synthetic peptide 31-43 as a specific immunological activator in CD and demonstrate its capability to stimulate production/secretion of CD-specific antibodies. We envision that this organ culture system may prove to be useful as a new technique for CD diagnosis.
当在麦醇溶蛋白肽存在的情况下进行培养时，处于临床缓解期的乳糜泻（CD）患者的肠黏膜可在体外产生抗肌内膜抗体和抗转谷氨酰胺酶抗体。我们的目的是利用这种器官培养系统作为一种手段，来检测培养上清液中乳糜泻（CD）的特征性抗体。在三种不同激活剂存在的情况下进行器官培养，以评估哪一种激活剂能在CD临床缓解期患者的肠黏膜中诱导最强的抗体反应。我们的数据证实了合成肽31 - 43作为CD中一种特异性免疫激活剂的高效性，并证明了其刺激产生/分泌CD特异性抗体的能力。我们设想这种器官培养系统可能被证明是一种用于CD诊断的新技术。
