化脓性链球菌次要菌毛蛋白FctB的纯化、结晶及初步晶体学分析
Purification, crystallization and preliminary crystallographic analysis of the minor pilin FctB from Streptococcus pyogenes.
作者信息
Linke Christian, Young Paul G, Kang Hae Joo, Proft Thomas, Baker Edward N
机构信息
School of Biological Sciences, University of Auckland, Auckland, New Zealand.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Feb 1;66(Pt 2):177-9. doi: 10.1107/S1744309109051951. Epub 2010 Jan 28.
Abstract
The minor pilin FctB is an integral part of the pilus assembly expressed by Streptococcus pyogenes. Since it is located at the cell wall, it can be hypothesized that it functions as a cell-wall anchor for the streptococcal pilus. In order to elucidate its structure, the genes for FctB from the S. pyogenes strains 90/306S and SF370 were cloned for overexpression in Escherichia coli. FctB from strain 90/306S was crystallized by the sitting-drop vapour-diffusion method using sodium citrate as a precipitant. The hexagonal FctB crystals belonged to space group P6(1) or P6(5), with unit-cell parameters a = b = 95.15, c = 100.25 A, and diffracted to 2.9 A resolution.
摘要
次要菌毛蛋白FctB是化脓性链球菌表达的菌毛组装的一个组成部分。由于它位于细胞壁，因此可以推测它作为链球菌菌毛的细胞壁锚定物发挥作用。为了阐明其结构，将化脓性链球菌菌株90/306S和SF370的FctB基因进行克隆，以便在大肠杆菌中过表达。采用坐滴气相扩散法，以柠檬酸钠作为沉淀剂，使菌株90/306S的FctB结晶。六边形的FctB晶体属于空间群P6(1)或P6(5)，晶胞参数a = b = 95.15，c = 100.25 Å，衍射分辨率达到2.9 Å。
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