Nakamura Megumi, Sato Eiji, Miura Tomoyuki, Baba Kenji, Shimoda Tetsuya, Miyazawa Takayuki
Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, Japan.
J Vet Med Sci. 2010 Jun;72(6):787-90. doi: 10.1292/jvms.09-0214. Epub 2010 Feb 2.
Feline leukemia virus (FeLV) is classified into three receptor interference subgroups, A, B and C. In this study, to differentiate FeLV subgroups, we developed a simple assay system using pseudotype viruses expressing green fluorescent protein (GFP). We prepared gfp pseudotype viruses, named gfp(FeLV-A), gfp(FeLV-B) and gfp(FeLV-C) harboring envelopes of FeLV-A, B and C, respectively. The gfp pseudotype viruses completely interfered with the same subgroups of FeLV reference strains on FEA cells (a feline embryonic fibroblast cell line). We also confirmed that the pseudotype viruses could differentiate FeLV subgroups in field isolates. The assay will be useful for differential diagnosis of FeLV subgroups in veterinary diagnostic laboratories in the future.
猫白血病病毒（FeLV）分为三个受体干扰亚组，A、B和C。在本研究中，为了区分FeLV亚组，我们开发了一种使用表达绿色荧光蛋白（GFP）的假型病毒的简单检测系统。我们制备了gfp假型病毒，分别命名为gfp(FeLV-A)、gfp(FeLV-B)和gfp(FeLV-C)，它们分别携带FeLV-A、B和C的包膜。这些gfp假型病毒在FEA细胞（一种猫胚胎成纤维细胞系）上完全干扰了相同亚组的FeLV参考毒株。我们还证实了这些假型病毒可以区分野外分离株中的FeLV亚组。该检测方法未来将有助于兽医诊断实验室对FeLV亚组进行鉴别诊断。
