Department of Surgery and Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
Pancreas. 2010 Jul;39(5):646-52. doi: 10.1097/MPA.0b013e3181c72baf.
To investigate the effects and mechanism of Frondanol-A5P, a polar extract from Cucumaria frondosa, on growth inhibition and apoptosis in S2013 and AsPC-1 human pancreatic cancer cells.
The effects of Frondanol-A5P on proliferation, cell cycle, expression of cell cycle proteins and p21, phosphorylation of MAP kinases, annexin V binding, and caspase-3 activation were examined.
Frondanol-A5P inhibited proliferation and induced G2/M phase cell cycle arrest in both cell lines with decreased expression of cyclin A, cyclin B, and cdc25c. Frondanol-A5P induced phosphorylation of stress-activated protein kinase and Janus kinase (SAPK/JAK) and p38 mitogen-activated protein kinase (MAP) within 5 minutes. Frondanol-A5P markedly increased expression of p21 messenger RNA and protein at 3 hours in both cell lines. This effect was reduced by the p38 kinase inhibitor, SB203580. Frondanol-A5P markedly increased annexin V binding and activated caspase-3.
Frondanol-A5 causes cell cycle arrest and apoptosis in human pancreatic cancer cells. These changes are associated with decreased expression of cyclin A, cyclin B, and cdc25c and increased expression of p21 that, at least in part, is mediated by a p38 kinase-dependent mechanism. Because Frondanol-A5P is derived from an edible, nontoxic, sea cucumber, it may be valuable for nutritional therapy or prevention of pancreatic cancer.
研究 Frondanol-A5P（一种来自黄瓜鱼的极性提取物）对 S2013 和 AsPC-1 人胰腺癌细胞生长抑制和凋亡的影响及其作用机制。
检测 Frondanol-A5P 对增殖、细胞周期、细胞周期蛋白表达和 p21、MAP 激酶磷酸化、膜联蛋白 V 结合和 caspase-3 激活的影响。
Frondanol-A5P 抑制两种细胞系的增殖并诱导 G2/M 期细胞周期阻滞，同时降低 cyclin A、cyclin B 和 cdc25c 的表达。Frondanol-A5P 在 5 分钟内诱导应激激活蛋白激酶和 Janus 激酶（SAPK/JAK）和 p38 丝裂原激活蛋白激酶（MAP）的磷酸化。Frondanol-A5P 在两种细胞系中均在 3 小时显著增加 p21 信使 RNA 和蛋白的表达。该作用可被 p38 激酶抑制剂 SB203580 减弱。Frondanol-A5P 显著增加膜联蛋白 V 结合并激活 caspase-3。
Frondanol-A5 导致人胰腺癌细胞周期阻滞和凋亡。这些变化与 cyclin A、cyclin B 和 cdc25c 的表达降低以及 p21 的表达增加有关，至少部分通过 p38 激酶依赖性机制介导。由于 Frondanol-A5P 来源于一种可食用的、无毒的海参，因此它可能对营养治疗或预防胰腺癌有价值。
