Department of Cell and Molecular Physiology, McAllister Heart Institute, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7545, USA.
J Cereb Blood Flow Metab. 2010 May;30(5):923-34. doi: 10.1038/jcbfm.2010.10. Epub 2010 Feb 3.
Severity of stroke varies widely among individuals. Whether differences in the extent of the native (preexisting) pial collateral circulation exist and contribute to this variability is unknown. We addressed these questions and probed for potential genetic contributions using morphometric analysis of the collateral circulation in 15 inbred mouse strains recently shown to exhibit wide differences in infarct volume. Morphometrics were determined in the unligated left hemisphere (for native collaterals) and ligated right hemisphere (for remodeled collaterals) 6 days after permanent middle cerebral artery (MCA) occlusion. Variation among strains in native collateral number, diameter, MCA, anterior cerebral artery (ACA), and posterior cerebral artery (PCA) tree territories were, respectively: 56-fold, 3-fold, 42%, 56%, and 61%. Collateral length (P<0.001) and the number of penetrating arterioles branching from them also varied (P<0.05). Infarct volume correlated inversely with collateral number (P<0.0001), diameter (P<0.0001), and penetrating arteriole number (P<0.05) and directly with MCA territory (P<0.05). Relative collateral conductance and MCA territory, when factored together, strongly predicted infarct volume (P<0.0001). Outward remodeling of collaterals in the ligated hemisphere varied approximately 3-fold. These data show that the extent of the native pial collateral circulation and collateral remodeling after obstruction vary widely with genetic background, and suggest that this variability, due to natural polymorphisms, is a major contributor to variability in infarct volume.
脑卒中的严重程度在个体之间差异很大。是否存在并导致这种变异性的固有（预先存在的）软脑膜侧支循环程度的差异尚不清楚。我们使用最近显示在梗死体积方面存在广泛差异的 15 个近交系小鼠品系的侧支循环形态计量分析来解决这些问题，并探讨潜在的遗传贡献。在永久性大脑中动脉 (MCA) 闭塞后 6 天，对未结扎的左侧半球（用于固有侧支）和结扎的右侧半球（用于重塑侧支）进行形态计量学测定。在固有侧支数量、直径、MCA、大脑前动脉 (ACA) 和大脑后动脉 (PCA) 树区之间，品系间的差异分别为：56 倍、3 倍、42%、56%和 61%。侧支长度（P<0.001）和从它们分支的穿透小动脉数量也有所不同（P<0.05）。梗死体积与侧支数量（P<0.0001）、直径（P<0.0001）和穿透小动脉数量（P<0.05）呈负相关，与 MCA 区呈正相关（P<0.05）。当考虑到相对侧支导纳和 MCA 区时，它们强烈预测梗死体积（P<0.0001）。结扎半球侧支的外向重塑大约变化了 3 倍。这些数据表明，固有软脑膜侧支循环的程度和阻塞后的侧支重塑在很大程度上因遗传背景而异，并且表明这种由于自然多态性引起的变异性是梗死体积变异性的主要原因。
