高速线扫描共焦成像刺激诱发的视网膜内固有光学信号。
High-speed line-scan confocal imaging of stimulus-evoked intrinsic optical signals in the retina.
机构信息
Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA.
出版信息
Opt Lett. 2010 Feb 1;35(3):426-8. doi: 10.1364/OL.35.000426.
Abstract
A rapid line-scan confocal imager was developed for functional imaging of the retina. In this imager, an acousto-optic deflector was employed to produce mechanical vibration- and inertia-free light scanning, and a high-speed (68,000 Hz) linear CCD camera was used to achieve subcellular and submillisecond spatiotemporal resolution imaging. Two imaging modalities, i.e., frame-by-frame and line-by-line recording, were validated for the reflected light detection of intrinsic optical signals (IOSs) in visible light stimulus activated frog retinas. Experimental results indicated that fast IOSs were tightly correlated with retinal stimuli and could track visible light flicker stimulus frequency up to at least 2 Hz.
摘要
我们研制了一种用于视网膜功能成像的快速线扫描共聚焦成像仪。在该成像仪中，采用声光偏转器进行无机械振动和惯性的光扫描，并用高速（68000Hz）线阵 CCD 相机实现亚细胞和亚毫秒时空分辨率成像。我们对两种成像模式（帧频和行频记录）进行了验证，以检测可见光刺激激活的青蛙视网膜的固有光信号（IOS）的反射光。实验结果表明，快速 IOS 与视网膜刺激紧密相关，并且可以跟踪可见光闪烁刺激频率，最高可达至少 2Hz。
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