体内共聚焦固有光学信号定位视网膜功能障碍。
In vivo confocal intrinsic optical signal identification of localized retinal dysfunction.
机构信息
Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, Alabama, USA.
出版信息
Invest Ophthalmol Vis Sci. 2012 Dec 13;53(13):8139-45. doi: 10.1167/iovs.12-10732.
PURPOSE
The purposes of this study were to investigate the physiological mechanism of stimulus-evoked fast intrinsic optical signals (IOSs) recorded in dynamic confocal imaging of the retina, and to demonstrate the feasibility of in vivo confocal IOS mapping of localized retinal dysfunctions.
METHODS
A rapid line-scan confocal ophthalmoscope was constructed to achieve in vivo confocal IOS imaging of frog (Rana pipiens) retinas at cellular resolution. In order to investigate the physiological mechanism of confocal IOS, comparative IOS and electroretinography (ERG) measurements were made using normal frog eyes activated by variable-intensity stimuli. A dynamic spatiotemporal filtering algorithm was developed to reject the contamination of hemodynamic changes on fast IOS recording. Laser-injured frog eyes were employed to test the potential of confocal IOS mapping of localized retinal dysfunctions.
RESULTS
Comparative IOS and ERG experiments revealed a close correlation between the confocal IOS and retinal ERG, particularly the ERG a-wave, which has been widely used to evaluate photoreceptor function. IOS imaging of laser-injured frog eyes indicated that the confocal IOS could unambiguously detect localized (30 μm) functional lesions in the retina before a morphological abnormality is detectable.
CONCLUSIONS
The confocal IOS predominantly results from retinal photoreceptors, and can be used to map localized photoreceptor lesion in laser-injured frog eyes. We anticipate that confocal IOS imaging can provide applications in early detection of age-related macular degeneration, retinitis pigmentosa, and other retinal diseases that can cause pathological changes in the photoreceptors.
目的
本研究旨在探讨在视网膜动态共焦成像中记录的刺激诱发的快速固有光学信号（IOS）的生理机制，并演示在体共焦 IOS 映射局部视网膜功能障碍的可行性。
方法
构建了一种快速线扫描共焦眼底镜，以实现对青蛙（Rana pipiens）视网膜的在体共焦 IOS 成像，达到细胞分辨率。为了研究共焦 IOS 的生理机制，使用可变强度刺激激活正常青蛙眼，进行了共焦 IOS 和视网膜电图（ERG）的比较测量。开发了一种动态时空滤波算法，以消除对快速 IOS 记录的血液动力学变化的污染。使用激光损伤的青蛙眼测试了局部视网膜功能障碍的共焦 IOS 映射的潜力。
结果
比较 IOS 和 ERG 实验表明，共焦 IOS 与视网膜 ERG 密切相关，特别是 ERG 的 a 波，它已被广泛用于评估光感受器的功能。激光损伤的青蛙眼的 IOS 成像表明，在形态异常可检测之前，共焦 IOS 可以明确地检测到视网膜中的局部（30μm）功能损伤。
结论
共焦 IOS 主要来源于视网膜光感受器，可用于映射激光损伤的青蛙眼中的局部光感受器损伤。我们预计共焦 IOS 成像可应用于年龄相关性黄斑变性、色素性视网膜炎和其他可引起光感受器病理变化的视网膜疾病的早期检测。
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