微透镜阵列记录局部视网膜反应。
Microlens array recording of localized retinal responses.
机构信息
Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA.
出版信息
Opt Lett. 2010 Nov 15;35(22):3838-40. doi: 10.1364/OL.35.003838.
Abstract
We designed a rapid functional imager for the parallel recording of localized intrinsic optical signals (IOSs). This imager used a microlens array (MLA)-based illuminator to deliver visible stimulus light and near-infrared (NIR) recording light simultaneously. The parfocal configuration of the stimulus and recording light illumination enabled confocal recording of the stimulus-evoked IOSs. Because the MLA stimulation/recording spots were widely separated on the retina, and only the photoreceptors within the MLA stimulation/recording spots were stimulated, the potential IOS cross talk effect among neighboring retinal areas was minimized. Our experiments revealed robust IOS activities tightly correlated with localized retinal responses.
摘要
我们设计了一种用于并行记录局部固有光学信号（IOSs）的快速功能成像仪。该成像仪使用基于微透镜阵列（MLA）的照明器同时传输可见刺激光和近红外（NIR）记录光。刺激光和记录光照明的共焦配置实现了对刺激引起的 IOS 的共焦记录。由于 MLA 刺激/记录点在视网膜上广泛分离，并且只有在 MLA 刺激/记录点内的光感受器受到刺激，因此相邻视网膜区域之间的潜在 IOS 串扰效应最小化。我们的实验揭示了与局部视网膜反应紧密相关的强大 IOS 活动。
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