Unidade Clínica de Miocardiopatias - Instituto do Coração - Hospital das Clinicas, Faculdade de Medicina da Universidade de São Paulo,São Paulo/SP,Brazil.
Clinics (Sao Paulo). 2010;65(1):23-7. doi: 10.1590/S1807-59322010000100005.
Obesity increases triglyceride levels and decreases high-density lipoprotein concentrations in plasma. Artificial emulsions resembling lipidic plasma lipoprotein structures have been used to evaluate low-density lipoprotein metabolism. In grade III obesity, low density lipoprotein metabolism is poorly understood.
To evaluate the kinetics with which a cholesterol-rich emulsion (called a low-density emulsion) binds to low-density lipoprotein receptors in a group of patients with grade III obesity by the fractional clearance rate.
A low-density emulsion was labeled with [(14)C]-cholesterol ester and [(3)H]-triglycerides and injected intravenously into ten normolipidemic non-diabetic patients with grade III obesity [body mass index higher than 40 kg/m(2)] and into ten non-obese healthy controls. Blood samples were collected over 24 hours to determine the plasma decay curve and to calculate the fractional clearance rate.
There was no difference regarding plasma levels of total cholesterol or low-density lipoprotein cholesterol between the two groups. The fractional clearance rate of triglycerides was 0.086 +/- 0.044 in the obese group and 0.122 +/- 0.026 in the controls (p = 0.040), and the fractional clearance rate of cholesterol ester (h(-1)) was 0.052 +/- 0.021 in the obese subjects and 0.058 +/- 0.015 (p = 0.971) in the controls.
Grade III obese subjects exhibited normal low-density lipoprotein removal from plasma as tested by the nanoemulsion method, but triglyceride removal was slower.
肥胖会增加血浆中的甘油三酯水平并降低高密度脂蛋白浓度。人工乳液类似于脂质血浆脂蛋白结构，已被用于评估低密度脂蛋白代谢。在三级肥胖中，对低密度脂蛋白代谢的了解甚少。
通过分数清除率评估一组三级肥胖患者的富含胆固醇的乳液（称为低密度乳液）与低密度脂蛋白受体结合的动力学。
将 [(14)C]-胆固醇酯和 [(3)H]-甘油三酯标记的低密度乳液静脉内注射到十名非糖尿病的三级肥胖症患者（体重指数大于 40 kg/m(2)）和十名非肥胖的健康对照者中。采集 24 小时的血液样本以确定血浆衰减曲线并计算分数清除率。
两组患者的总胆固醇或低密度脂蛋白胆固醇的血浆水平无差异。肥胖组的甘油三酯分数清除率为 0.086 +/- 0.044，对照组为 0.122 +/- 0.026（p = 0.040），胆固醇酯的分数清除率（h(-1)）肥胖组为 0.052 +/- 0.021，对照组为 0.058 +/- 0.015（p = 0.971）。
用纳米乳液法测试，三级肥胖患者的低密度脂蛋白从血浆中的清除正常，但甘油三酯的清除较慢。
