利用微阵列分析对不育男性和可育供体精子之间转录差异的本体论评估。
Ontological evaluation of transcriptional differences between sperm of infertile males and fertile donors using microarray analysis.
机构信息
Instituto Universitario IVI Valencia, Universidad de Valencia, Valencia, Spain.
出版信息
J Assist Reprod Genet. 2010 Feb;27(2-3):111-20. doi: 10.1007/s10815-010-9388-5. Epub 2010 Feb 2.
Abstract
PURPOSE
To catalogue Gene Ontology terms in the sperm of infertile human males vs. donors of proven fertility by analyzing five samples from each of the two groups (five aliquots from fresh sperm and five post-swim-up).
METHODS
Microarray technology was employed to study the mRNA profile of both fresh and post-swim-up pooled samples from infertile males and donors.
RESULTS
Genes that were differentially expressed in the two populations and expressed in only one of two were analyzed to determine the gene products in terms of their associated Gene Ontology terms. Each group presented a different number and pattern of Gene Ontology terms.
CONCLUSIONS
We found differences in Gene Ontology terms between the two groups. These differences could potentially be employed to establish markers of fertility success and to identify cellular processes and complex systems related with male infertility.
摘要
目的
通过分析两组各五个样本（新鲜精子的五个等分和游泳后五个等分），对不育男性和可育供体的精子中的基因本体论术语进行编目。
方法
采用微阵列技术研究不育男性和供体的新鲜和游泳后混合样本的 mRNA 图谱。
结果
对两个群体中差异表达且仅在两个群体之一中表达的基因进行分析，以确定与基因本体论术语相关的基因产物。每个组呈现出不同数量和模式的基因本体论术语。
结论
我们发现两组之间的基因本体论术语存在差异。这些差异可能被用于建立生育成功的标志物，并识别与男性不育相关的细胞过程和复杂系统。
相似文献
1
Ontological evaluation of transcriptional differences between sperm of infertile males and fertile donors using microarray analysis.利用微阵列分析对不育男性和可育供体精子之间转录差异的本体论评估。
J Assist Reprod Genet. 2010 Feb;27(2-3):111-20. doi: 10.1007/s10815-010-9388-5. Epub 2010 Feb 2.
2
Microarray analysis in sperm from fertile and infertile men without basic sperm analysis abnormalities reveals a significantly different transcriptome.对无基本精子分析异常的生育能力正常和不育男性的精子进行微阵列分析，结果显示转录组存在显著差异。
Fertil Steril. 2009 Apr;91(4 Suppl):1307-10. doi: 10.1016/j.fertnstert.2008.01.078. Epub 2008 Mar 25.
3
Differential transcriptomic profile in spermatozoa achieving pregnancy or not via ICSI.通过 ICSI 实现妊娠或未实现妊娠的精子中的差异转录组谱。
Reprod Biomed Online. 2011 Jan;22(1):25-36. doi: 10.1016/j.rbmo.2010.09.013. Epub 2010 Nov 30.
4
Impact of male fertility status on the transcriptome of the bovine epididymis.雄性生育状态对牛附睾转录组的影响。
Mol Hum Reprod. 2017 Jun 1;23(6):355-369. doi: 10.1093/molehr/gax019.
5
Sperm transcriptome profiling in oligozoospermia.少精子症的精子转录组谱分析。
J Assist Reprod Genet. 2012 Jan;29(1):3-10. doi: 10.1007/s10815-011-9644-3. Epub 2011 Oct 12.
6
Differential Genes Expression between Fertile and Infertile Spermatozoa Revealed by Transcriptome Analysis.转录组分析揭示的可育与不育精子之间的差异基因表达
PLoS One. 2015 May 14;10(5):e0127007. doi: 10.1371/journal.pone.0127007. eCollection 2015.
7
Spermatozoa from patients with seminal alterations exhibit a differential micro-ribonucleic acid profile.精液异常患者的精子表现出不同的微小核糖核酸谱。
Fertil Steril. 2015 Sep;104(3):591-601. doi: 10.1016/j.fertnstert.2015.06.015. Epub 2015 Jul 2.
8
Spermatozoa from normozoospermic fertile and infertile individuals convey a distinct miRNA cargo.来自正常精子生育力正常和不育个体的精子携带独特的微小RNA（miRNA）负载。
Andrology. 2016 Nov;4(6):1028-1036. doi: 10.1111/andr.12276. Epub 2016 Sep 27.
9
Human sperm Toll-like receptor 4 (TLR4) mediates acrosome reaction, oxidative stress markers, and sperm parameters in response to bacterial lipopolysaccharide in infertile men.人类精子中的Toll样受体4（TLR4）介导了不育男性精子对细菌脂多糖的顶体反应、氧化应激标志物及精子参数。
J Assist Reprod Genet. 2017 Aug;34(8):1067-1077. doi: 10.1007/s10815-017-0957-8. Epub 2017 May 26.
10
The transcriptome of spermatozoa used in homologous intrauterine insemination varies considerably between samples that achieve pregnancy and those that do not.用于同源宫腔内授精的精子转录组在妊娠样本和非妊娠样本之间差异很大。
Fertil Steril. 2010 Sep;94(4):1360-1373. doi: 10.1016/j.fertnstert.2009.07.1671. Epub 2009 Sep 30.
引用本文的文献
1
Human RNAs as potential biomarkers for sperm quality and male infertility.人类RNA作为精子质量和男性不育的潜在生物标志物。
J Biomed Res. 2024 May 25;38(5):1-12. doi: 10.7555/JBR.37.20230296.
2
Differential sperm proteomic profiles according to pregnancy achievement in intracytoplasmic sperm injection cycles: a pilot study.卵胞浆内单精子注射周期中根据妊娠结局的差异精子蛋白质组学图谱：一项初步研究。
J Assist Reprod Genet. 2021 Jun;38(6):1507-1521. doi: 10.1007/s10815-021-02098-0. Epub 2021 Apr 9.
3
Genetic and genomic characterization of vulva size traits in Yorkshire and Landrace gilts.约克夏和长白母猪外阴大小性状的遗传和基因组特征分析。
BMC Genet. 2020 Mar 12;21(1):28. doi: 10.1186/s12863-020-0834-9.
4
Array CGH-based detection of CNV regions and their potential association with reproduction and other economic traits in Holsteins.基于阵列比较基因组杂交的 CNV 区域检测及其与荷斯坦奶牛繁殖和其他经济性状的潜在关联。
BMC Genomics. 2019 Mar 7;20(1):181. doi: 10.1186/s12864-019-5552-1.
5
Sperm lipidic profiles differ significantly between ejaculates resulting in pregnancy or not following intracytoplasmic sperm injection.经胞浆内单精子注射后导致妊娠和未导致妊娠的精子脂质谱存在显著差异。
J Assist Reprod Genet. 2018 Nov;35(11):1973-1985. doi: 10.1007/s10815-018-1284-4. Epub 2018 Aug 14.
6
Gonadal transcriptomics elucidate patterns of adaptive evolution within marine rockfishes (Sebastes).性腺转录组学揭示了海洋岩鱼（Sebastes）的适应性进化模式。
BMC Genomics. 2015 Sep 2;16(1):656. doi: 10.1186/s12864-015-1870-0.
7
OMICS: Current and future perspectives in reproductive medicine and technology.组学：生殖医学与技术的现状与未来展望
J Hum Reprod Sci. 2014 Apr;7(2):73-92. doi: 10.4103/0974-1208.138857.
8
The presence, role and clinical use of spermatozoal RNAs.精子 RNA 的存在、作用和临床应用。
Hum Reprod Update. 2013 Nov-Dec;19(6):604-24. doi: 10.1093/humupd/dmt031. Epub 2013 Jul 14.
9
Inferring quantitative trait pathways associated with bull fertility from a genome-wide association study.从全基因组关联研究中推断与公牛生育力相关的数量性状途径。
Front Genet. 2013 Jan 11;3:307. doi: 10.3389/fgene.2012.00307. eCollection 2012.
10
Digital holographic microscopy in human sperm imaging.数字全息显微镜在人类精子成像中的应用。
J Assist Reprod Genet. 2011 Aug;28(8):725-9. doi: 10.1007/s10815-011-9584-y. Epub 2011 Jun 11.
本文引用的文献
1
Contribution of sperm molecular features to embryo quality and assisted reproduction success.精子分子特征对胚胎质量和辅助生殖成功的作用。
Reprod Biomed Online. 2008 Dec;17(6):855-65. doi: 10.1016/s1472-6483(10)60415-4.
2
Extracellular, circulating proteasomes and ubiquitin - incidence and relevance.细胞外循环蛋白酶体与泛素——发生率及相关性
Biochim Biophys Acta. 2008 Dec;1782(12):817-23. doi: 10.1016/j.bbadis.2008.06.005. Epub 2008 Jun 18.
3
The centrosome and early embryogenesis: clinical insights.中心体与早期胚胎发育：临床见解
Reprod Biomed Online. 2008 Apr;16(4):485-91. doi: 10.1016/s1472-6483(10)60455-5.
4
Paternal contributions: new functional insights for spermatozoal RNA.父系贡献：精子RNA的新功能见解
J Cell Biochem. 2008 Aug 1;104(5):1570-9. doi: 10.1002/jcb.21756.
5
Stem cell defects in ATM-deficient undifferentiated spermatogonia through DNA damage-induced cell-cycle arrest.通过DNA损伤诱导的细胞周期停滞，ATM缺陷型未分化精原细胞中的干细胞缺陷。
Cell Stem Cell. 2008 Feb 7;2(2):170-82. doi: 10.1016/j.stem.2007.10.023.
6
Microarray analysis in sperm from fertile and infertile men without basic sperm analysis abnormalities reveals a significantly different transcriptome.对无基本精子分析异常的生育能力正常和不育男性的精子进行微阵列分析，结果显示转录组存在显著差异。
Fertil Steril. 2009 Apr;91(4 Suppl):1307-10. doi: 10.1016/j.fertnstert.2008.01.078. Epub 2008 Mar 25.
7
Bioinformatics applications for pathway analysis of microarray data.用于微阵列数据通路分析的生物信息学应用。
Curr Opin Biotechnol. 2008 Feb;19(1):50-4. doi: 10.1016/j.copbio.2007.11.005. Epub 2008 Jan 22.
8
MUC1 in human testis and ejaculated spermatozoa and its relationship to male fertility status.
Fertil Steril. 2008 Aug;90(2):450-2. doi: 10.1016/j.fertnstert.2007.06.104. Epub 2007 Oct 23.
9
Energy metabolism and sperm function.能量代谢与精子功能。
Soc Reprod Fertil Suppl. 2007;65:309-25.
10
A possible role for sperm RNA in early embryo development.精子RNA在早期胚胎发育中的潜在作用。
Theriogenology. 2007 Sep 1;68 Suppl 1:S147-55. doi: 10.1016/j.theriogenology.2007.05.058. Epub 2007 Jun 21.
Zotero 插件