利用微阵列分析对不育男性和可育供体精子之间转录差异的本体论评估。
Ontological evaluation of transcriptional differences between sperm of infertile males and fertile donors using microarray analysis.
机构信息
Instituto Universitario IVI Valencia, Universidad de Valencia, Valencia, Spain.
出版信息
J Assist Reprod Genet. 2010 Feb;27(2-3):111-20. doi: 10.1007/s10815-010-9388-5. Epub 2010 Feb 2.
Abstract
PURPOSE
To catalogue Gene Ontology terms in the sperm of infertile human males vs. donors of proven fertility by analyzing five samples from each of the two groups (five aliquots from fresh sperm and five post-swim-up).
METHODS
Microarray technology was employed to study the mRNA profile of both fresh and post-swim-up pooled samples from infertile males and donors.
RESULTS
Genes that were differentially expressed in the two populations and expressed in only one of two were analyzed to determine the gene products in terms of their associated Gene Ontology terms. Each group presented a different number and pattern of Gene Ontology terms.
CONCLUSIONS
We found differences in Gene Ontology terms between the two groups. These differences could potentially be employed to establish markers of fertility success and to identify cellular processes and complex systems related with male infertility.
摘要
目的
通过分析两组各五个样本（新鲜精子的五个等分和游泳后五个等分），对不育男性和可育供体的精子中的基因本体论术语进行编目。
方法
采用微阵列技术研究不育男性和供体的新鲜和游泳后混合样本的 mRNA 图谱。
结果
对两个群体中差异表达且仅在两个群体之一中表达的基因进行分析，以确定与基因本体论术语相关的基因产物。每个组呈现出不同数量和模式的基因本体论术语。
结论
我们发现两组之间的基因本体论术语存在差异。这些差异可能被用于建立生育成功的标志物，并识别与男性不育相关的细胞过程和复杂系统。
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