Department of Gynecology and Obstetrics, Faculty of Medicine, Masaryk University, and Faculty Hospital, Brno, Czech Republic.
J Assist Reprod Genet. 2011 Aug;28(8):725-9. doi: 10.1007/s10815-011-9584-y. Epub 2011 Jun 11.
The aim of this study was to use digital holographic microscopy (DHM) in human sperm imaging and compare quantitative phase contrast of sperm heads in normozoospermia (NZ) and oligoasthenozoospermia (OAT).
DHM spermatozoa imaging and repeated quantitative phase shift evaluation were used. Five NZ and 5 OAT samples were examined. Semen samples were examined by semen analysis and processed for DHM. Main outcome measures were maximum phase shift value of the sperm heads. Differences of the phase shift and in NZ and OAT samples were statistically tested.
In NZ samples median phase shifts were in the range 2.72-3.21 rad and 2.00-2.15 in OAT samples. Differences among individual samples were statistically significant (p < 0.001) in both groups. Median phase shift according to sperm count was 2.90 rad in NZ samples and 2.00 rad in OAT samples. This difference was statistically significant (p < 0.001).
Quantitative evaluation of the phase shift by DHM could provide new information on the exact structure and composition of the sperm head. At present, this technique is not established for clinical utility.
本研究旨在使用数字全息显微镜（DHM）对人精子进行成像，并比较正常精子症（NZ）和少精子症（OAT）精子头部的定量相位对比。
使用 DHM 精子成像和重复定量相移评估。检查了 5 个 NZ 和 5 个 OAT 样本。精液样本通过精液分析进行检查，并进行 DHM 处理。主要观察指标为精子头部的最大相移值。统计检验了 NZ 和 OAT 样本中相移的差异。
在 NZ 样本中，中位数相移范围为 2.72-3.21 拉德，在 OAT 样本中为 2.00-2.15 拉德。两组中个体样本之间的差异均具有统计学意义（p<0.001）。根据精子计数，NZ 样本中的中位数相移为 2.90 拉德，OAT 样本中的中位数相移为 2.00 拉德。这种差异具有统计学意义（p<0.001）。
DHM 对相移的定量评估可以提供关于精子头的确切结构和组成的新信息。目前，该技术尚未在临床应用中得到确立。
