Department of Pathology, Tifton Veterinary Diagnostic and Investigational Laboratory, College of Veterinary Medicine, University of Georgia, 43 Brighton Rd, Tifton, GA, 31793, USA.
Vet Res Commun. 2010 Feb;34(2):153-60. doi: 10.1007/s11259-010-9341-1. Epub 2010 Feb 3.
Mast cell count (MCC) in 45 dogs with cutaneous hemangioma (HA, n = 12), hemangiosarcoma (HSA, n = 12), mammary adenoma (AD, n = 9) and mammary adenocarcinoma (AC, n = 12) was made using Toluidine blue stained sections. Antibodies against endothelial cell markers, Factor VIII and VEGF were used to visualize and determine the hot spot micro-vessel density (MVD). Total MCC and MCC along the invasive edges were significantly higher (p < 0.001) in canine mammary AC than in AD. The total MCC did not significantly differ (p > 0.05), in HSAs (8.6 +/- 3.3) than in HAs (5.5 +/- 2.8). There is a positive correlation (r = 0.14) between the hot spot MCC and MVD in mammary AC, although not significant (p = 0.3172), indicating that mast cells are associated with angiogenesis in canine mammary AC. This study suggests that mast cells may play an important role in neovascularization of canine cutaneous vascular and mammary neoplasms. Detailed studies encompassing correlation of MCC and MVD with clinical outcomes and prognosis in these neoplasms are recommended.
采用甲苯胺蓝染色切片对 45 只患有皮肤血管瘤（HA，n = 12）、血管肉瘤（HSA，n = 12）、乳腺腺瘤（AD，n = 9）和乳腺腺癌（AC，n = 12）的犬进行了肥大细胞计数（MCC）。使用针对内皮细胞标志物、VIII 因子和 VEGF 的抗体来可视化和确定热点微血管密度（MVD）。犬乳腺 AC 的总 MCC 和沿侵袭边缘的 MCC 明显高于 AD（p < 0.001）。总 MCC 在 HSAs（8.6 +/- 3.3）与 HAs（5.5 +/- 2.8）之间无显著差异（p > 0.05）。尽管相关性不显著（p = 0.3172），但乳腺 AC 中热点 MCC 与 MVD 之间存在正相关（r = 0.14），表明肥大细胞与犬乳腺 AC 的血管生成有关。本研究表明，肥大细胞可能在犬皮肤血管和乳腺肿瘤的新生血管形成中发挥重要作用。建议对这些肿瘤中的 MCC 和 MVD 与临床结果和预后的相关性进行详细研究。
