School of Biological Sciences, Seoul National University, Seoul, 151-742, Republic of Korea.
J Microbiol. 2009 Dec;47(6):746-52. doi: 10.1007/s12275-009-2720-z. Epub 2010 Feb 4.
Hepatitis B virus (HBV) genome replication requires the packaging of viral factors (pregenomic RNA and polymerase) as well as host factors, including heat shock proteins and protein kinase C. Previous reports have suggested that there are several unidentified host factors that affect this encapsidation step. In this study, we identified a new host factor, nucleophosmin (B23) that interacts with the HBV core protein 149 (Cpl49). We analyzed this factor using NHS-activated sepharose resin and MALDI-TOF MS. Using the BIAcore analysis system, we were also able to deduce that the B23.1 residues 259-294 were required for the interaction between Cpl49 and B23.1 in vitro.
乙型肝炎病毒 (HBV) 基因组复制需要包装病毒因子（前基因组 RNA 和聚合酶）以及宿主因子，包括热休克蛋白和蛋白激酶 C。先前的报告表明，有几个未识别的宿主因子影响这个包裹步骤。在这项研究中，我们鉴定了一个新的宿主因子核仁磷酸蛋白 (B23)，它与乙型肝炎病毒核心蛋白 149 (Cpl49) 相互作用。我们使用 NHS 激活的琼脂糖树脂和 MALDI-TOF MS 分析了这个因子。使用 BIAcore 分析系统，我们还能够推断出 B23.1 残基 259-294 是 Cpl49 和 B23.1 之间相互作用所必需的。
