[电针对记忆障碍大鼠海马行为学及细胞因子的影响]

[Effect of electroacupuncture on ethology and cytokines of hippocampus in rats with dysmnesy].

作者信息

Xu Ying, Zhang Zhi-Xiong, Shen Rong, Wang Xing-Yu, Li Yun

机构信息

Department of Physiology, Shanghai University of Chinese Medicine, Shanghai 201203, China.

出版信息

Zhen Ci Yan Jiu. 2007 Apr;32(2):88-92.

PMID:17650650

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) on learning and memory impairment and cytokines of hippocampus in aging rats induced by D-galactose for exploring its underlying mechanism in the treatment of dysmnesy.

METHODS

A total of 27 SD rats were randomly divided into control group (n = 9), model group (n = 8) and EA group (n = 10). Dysmnesy model was induced by intraperitoneal injection of D-galactose. EA (3 Hz, continuous waves, 1 mA) was applied to "Baihui" (GV 20) and "Zusanli" (ST 36) for 20 min every time and on alternate days, continuously for 21 days. Morris water maze tests were conducted to detect the rat's escape latency, percentage of swimming distance in the original platform quadrant and the total distance (SD/TD) and the percentage of swimming time in the platform quadrant after removal of the platform (spatial probe test) which were used for assessing the animals' learning and memory ability. The contents of IL-1beta, IL-6 and TNF-alpha in the hippocampus tissue were assayed by radioimmunoassay.

RESULTS

Location navigation test showed that the escape latencies on the 2nd day and the 3rd day in model group were significantly longer than those of control group (P < 0.05), and the percents of SD/TD and swimming time in the platform quadrant were significantly lower than those of control group (P < 0.05, P < 0.01). In comparison with model group, the escape latency of EA group on the 3rd day was significantly shorter (P < 0.05), and the percent of SD/TD in the platform quadrant of EA group was significantly longer (P < 0.01). Compared with control group, IL-1beta and TNF-alpha contents in the hippocampus increased significantly in model group (P < 0.05, P < 0.01), while IL-6 content in model group decreased significantly (P < 0.01). In comparison with model group, the levels of IL-1beta and TNF-alpha in EA group decreased significantly (P < 0.05).

CONCLUSION

EA can improve the learning-memory ability in dysmnesy rats, which may be closely associated with its effects in regulating the levels of cytokines in the hippocampus.

摘要

目的

观察电针（EA）对D-半乳糖致衰老大鼠学习记忆障碍及海马细胞因子的影响，探讨其治疗记忆障碍的潜在机制。

方法

将27只SD大鼠随机分为对照组（n = 9）、模型组（n = 8）和电针组（n = 10）。通过腹腔注射D-半乳糖诱导记忆障碍模型。电针（3 Hz，连续波，1 mA）每次分别针刺“百会”（GV 20）和“足三里”（ST 36）20分钟，隔日1次，连续21天。进行Morris水迷宫试验，检测大鼠的逃避潜伏期、原平台象限游泳距离百分比和总距离（SD/TD）以及撤台后平台象限游泳时间百分比（空间探索试验），以评估动物的学习记忆能力。采用放射免疫法检测海马组织中IL-1β、IL-6和TNF-α的含量。

结果

定位航行试验显示，模型组第2天和第3天的逃避潜伏期明显长于对照组（P < 0.05），平台象限的SD/TD百分比和游泳时间百分比明显低于对照组（P < 0.05，P < 0.01）。与模型组相比，电针组第3天的逃避潜伏期明显缩短（P < 0.05），电针组平台象限的SD/TD百分比明显延长（P < 0.01）。与对照组相比，模型组海马中IL-1β和TNF-α含量明显升高（P < 0.05，P < 0.01），而模型组IL-6含量明显降低（P < 0.01）。与模型组相比，电针组IL-1β和TNF-α水平明显降低（P < 0.05）。