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利用频域荧光法解析柔性分子的构象分布和动力学

Resolution of the conformational distribution and dynamics of a flexible molecule using frequency-domain fluorometry.

作者信息

Lakowicz J R, Kuśba J, Wiczk W, Gryczynski I, Szmacinski H, Johnson M L

机构信息

University of Maryland, School of Medicine, Department of Biological Chemistry, Baltimore 21201.

出版信息

Biophys Chem. 1991 Jan;39(1):79-84. doi: 10.1016/0301-4622(91)85008-e.

DOI:10.1016/0301-4622(91)85008-e
PMID:2012836
Abstract

We report the first resolution of both the conformational distribution and end-to-end diffusion coefficient of a flexible molecule. This molecular information was recovered using only the donor intensity decay in a single solvent at a single viscosity, as observed by the technique of frequency-domain fluorometry. This technique can be extended to measurements of structural fluctuations of biological macromolecules.

摘要

我们报道了对柔性分子的构象分布和端到端扩散系数的首次解析。这些分子信息仅通过频域荧光测定技术，利用在单一溶剂、单一粘度下的供体强度衰减来获取。该技术可扩展到生物大分子结构波动的测量。

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