Torimiro Judith N, D'Arrigo Roberta, Takou Desire, Nanfack Aubin, Pizzi Daniele, Ngong Innocent, Carr Jean K, Joseph Fouda Pierre, Perno Carlo-Federico, Cappelli Giulia
Centre International de Référence "Chantal Biya", Yaounde, Cameroon.
New Microbiol. 2009 Oct;32(4):325-31.
A broad and rapidly changing HIV Type 1 (HIV-1) diversity has been reported from different populations in Cameroon since the early epidemic. Our understanding of HIV-1 dynamics can be improved by a systematic surveillance in Cameroon as accessibility and use of antiretroviral drugs increase. To contribute to this, we genotyped 30 samples by sequencing the protease and reverse transcriptase (proRT) genes of HIV-1. Phylogenetic analysis of the HIV-1 proRT sequences using the MEGA3 software showed that 26 (86.7%) were recombinant forms which included 20 (66.7%) circulating recombinant forms: CRF02_AG, (50%), CRF06_cpx (3.3%), CRF11 _cpx (10%) and CRF37_cpx (3.3%), and 6 unique recombinant forms (URF, 20%). Two of the six URFs were second generation recombinants and 4 contained unclassified segments. HIV-1 subtypes A1 (3.3%), C (3.3%) and D (6.7%) were also identified. Although partial sequences of HIV-1 genome were analysed, our results indicate that recombinant HIV-1 variants predominate in the AIDS epidemic in Cameroon. With the widespread use of antiretroviral drugs in Cameroon and the circulation of several HIV-1 variants within this population, the emergence of recombinants with unknown diagnostic and clinical consequences is a concern.
自艾滋病流行初期以来，喀麦隆不同人群中已报告了广泛且迅速变化的1型人类免疫缺陷病毒（HIV-1）多样性。随着抗逆转录病毒药物的可及性和使用增加，通过在喀麦隆进行系统监测，我们对HIV-1动态的理解可以得到改善。为促成此事，我们通过对HIV-1的蛋白酶和逆转录酶（proRT）基因进行测序，对30个样本进行了基因分型。使用MEGA3软件对HIV-1 proRT序列进行系统发育分析表明，26个（86.7%）为重组形式，其中包括20个（66.7%）循环重组形式：CRF02_AG（50%）、CRF06_cpx（3.3%）、CRF11_cpx（10%）和CRF37_cpx（3.3%），以及6个独特重组形式（URF，20%）。6个URF中有2个是第二代重组体，4个包含未分类片段。还鉴定出了HIV-1亚型A1（3.3%）、C（3.3%）和D（6.7%）。尽管分析了HIV-1基因组的部分序列，但我们的结果表明，重组HIV-1变体在喀麦隆的艾滋病流行中占主导地位。随着抗逆转录病毒药物在喀麦隆的广泛使用以及该人群中多种HIV-1变体的传播，具有未知诊断和临床后果的重组体的出现令人担忧。
