Division of Trauma, Surgical Critical Care, and Burns, Department of Surgery, University of California-San Diego School of Medicine, San Diego, CA, USA.
Mol Imaging. 2010 Feb;9(1):30-9.
Intestinal injury owing to inflammation, severe trauma, and burn is a leading cause of morbidity and mortality. Currently, animal models employed to study the intestinal response to injury and inflammation depend on outdated methods of analysis. Given that these classic intestinal assays are lethal to the experimental animal, there is no ability to study the gut response to injury in the same animal over time. We postulated that by developing an in vivo assay to image intestinal injury using fluorescent dye, it could complement other expensive, time-consuming, and semiquantitative classic means of detecting intestinal injury. We describe a novel in vivo, noninvasive method to image intestinal injury using a charge-coupled device (CCD) camera that allows for serial visual and quantitative analysis of intestinal injury. Our results correlate with traditional, time-consuming, semiquantitative assays of intestinal injury, now allowing the noninvasive, nonlethal assessment of injury over time.
肠道损伤是由炎症、严重创伤和烧伤引起的，是发病率和死亡率的主要原因。目前，用于研究肠道对损伤和炎症反应的动物模型依赖于过时的分析方法。鉴于这些经典的肠道检测方法对实验动物具有致命性，因此无法在同一动物身上随时间研究肠道对损伤的反应。我们假设通过开发一种使用荧光染料对肠道损伤进行成像的体内检测方法，可以补充其他昂贵、耗时和半定量的经典检测肠道损伤的方法。我们描述了一种使用电荷耦合器件 (CCD) 相机对肠道损伤进行体内、非侵入性成像的新方法，该方法允许对肠道损伤进行连续的视觉和定量分析。我们的结果与传统的、耗时的、半定量的肠道损伤检测方法相关，现在可以对损伤进行非侵入性、非致死性的随时间评估。
