Chen Haiyan, Wang Yunqing, Xu Jing, Ji Jinzi, Zhang Jian, Hu Yuzhu, Gu Yueqing
Department of Biomedical Engineering, School of Life Science and Technology, China Pharmaceutical University, 24 Shennong Road, Nanjing, 210009, People's Republic of China.
J Fluoresc. 2008 Sep;18(5):801-11. doi: 10.1007/s10895-007-0307-9. Epub 2008 Jan 5.
Near infrared CdHgTe quantum dots (QDs) acted as biomarker for in vivo imaging were synthesized in aqueous solution. The size and the fluorescence wavelength of the synthesized quantum dots can be arbitrary manipulated by using different refluxing time. In particular, the fluorescence wavelength was extended to near infrared range (700 approximately 900 nm), which make the in vivo imaging possible. Meanwhile, the characteristics, such as morphology, size, spectra, stability and toxicity were investigated. The dynamic bio-distribution, clearance from blood, liver and intestine in living animal were in vivo monitored by a NIR imaging system. The circulation of CdHgTe QDs in living mice was addressed semi-quantitatively according to the changes of fluorescence intensity. The high stability as well as high fluorescence intensity makes QDs particular interested candidates for in vivo imaging studies.
在水溶液中合成了用于体内成像的近红外CdHgTe量子点（QDs）。通过使用不同的回流时间，可以任意控制合成量子点的尺寸和荧光波长。特别地，荧光波长扩展到了近红外范围（700～900nm），这使得体内成像成为可能。同时，对其形貌、尺寸、光谱、稳定性和毒性等特性进行了研究。利用近红外成像系统对活体动物体内CdHgTe量子点的动态生物分布、从血液、肝脏和肠道的清除情况进行了监测。根据荧光强度的变化对活体小鼠体内CdHgTe量子点的循环进行了半定量分析。高稳定性以及高荧光强度使得量子点成为体内成像研究中特别受关注的候选物。
