Department of Surgery and Emory Critical Care Center, Emory University School of Medicine, Atlanta, GA, USA.
Department of Emergency and Critical Care Medicine, Chiba University Graduate School of Medicine, Chiba, Japan.
Methods Mol Biol. 2021;2321:169-175. doi: 10.1007/978-1-0716-1488-4_15.
Gut barrier function has been hypothesized to play a critical role in the pathophysiology of sepsis. Measuring intestinal permeability allows for a determination of barrier dysfunction under conditions of health and disease. Fluorescence-conjugated dyes such as fluorescein isothiocyanate-4 kDa dextran (FD4) have been commonly used for evaluating hyperpermeability. Here we describe a common method to measure gut permeability in vivo, following gavage with different sized dyes. In addition, we describe an ex vivo everted gut sac model that allows for discrimination of permeability by segmental geographic location along the intestine.
肠道屏障功能被认为在脓毒症的病理生理学中起着关键作用。测量肠道通透性可以确定在健康和疾病状态下的屏障功能障碍。荧光素异硫氰酸酯-4 kDa 葡聚糖（FD4）等荧光染料已被广泛用于评估高通透性。在这里，我们描述了一种使用不同大小染料经灌胃后测量体内肠道通透性的常用方法。此外，我们还描述了一种外翻肠囊模型，该模型允许根据肠道的节段地理位置来区分通透性。
