Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka, Japan.
J Biosci Bioeng. 2010 Feb;109(2):145-8. doi: 10.1016/j.jbiosc.2009.08.003. Epub 2009 Sep 9.
The amyloid beta protein with 42 amino acid residues (Abeta), which is a causative protein of Alzheimer's disease (AD), forms the complex with copper (II) to induce the cholesterol oxidase-like activity by the proton transfer from the cholesterol. In this study, the oxidation of cholesterol by Abeta/Cu complex was investigated on the surface of the zwitterionic phospholipid liposome including the bound water advantageous for the enhancement of the proton transfer. The bound water was pooled by the formation of cholesterol-rich domain within liposomes. The resulting reactivity was enhanced by the proton transfer mediated by the bound water.
含有 42 个氨基酸残基的淀粉样蛋白 β（Abeta）是阿尔茨海默病（AD）的致病蛋白，它与铜（II）形成复合物，通过胆固醇的质子转移诱导胆固醇氧化酶样活性。在这项研究中，在包含有利于质子转移增强的结合水的两性离子磷脂脂质体表面上研究了 Abeta/Cu 复合物对胆固醇的氧化作用。结合水通过在脂质体内部形成富含胆固醇的区域而聚集。通过结合水介导的质子转移，增强了所得反应性。
