Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P R China.
Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.
J Anim Sci. 2024 Jan 3;102. doi: 10.1093/jas/skae128.
LncRNAs (Long non-coding RNA) is an RNA molecule with a length of more than 200 bp. LncRNAs can directly act on mRNA, thus affecting the expression of downstream target genes and proteins, and widely participate in many important physiological and pathological regulation processes of the body. In this study, RNA-Seq was performed to detect lncRNAs from mammary gland tissues of three Chinese Holstein cows, including three cows at 7 d before calving and the same three cows at 30 d postpartum (early lactation stage). A total of 1,905 novel lncRNAs were detected, 57.3% of the predicted lncRNAs are ≥ 500 bp and 612 lncRNAs are intronic lncRNAs. The exon number of lncRNAs ranged from 2 to 10. A total of 96 lncRNAs were significantly differentially expressed between two stages, of which 47 were upregulated and 49 were downregulated. Pathway analysis found that target genes were mainly concentrated on the ECM-receptor interaction, Jak-STAT signaling pathway, PI3K-Akt signaling pathway, and TGF-beta signaling pathway. This study revealed the expression profile and characteristics of lncRNAs in the mammary gland tissues of Holstein cows at non-lactation and early lactation periods, and provided a basis for studying the functions of lncRNAs in Holstein cows during different lactation periods.
长链非编码 RNA（lncRNAs）是一种长度超过 200bp 的 RNA 分子。lncRNAs 可以直接作用于 mRNA，从而影响下游靶基因和蛋白质的表达，并广泛参与机体许多重要的生理和病理调节过程。本研究通过 RNA-Seq 技术检测了三头中国荷斯坦奶牛泌乳期前 7d 和泌乳期 30d（早期泌乳阶段）乳腺组织中的 lncRNAs。共检测到 1905 个新的 lncRNAs，预测的 lncRNAs 中 57.3%的长度大于 500bp，612 个 lncRNAs 为内含子 lncRNAs。lncRNAs 的外显子数量范围为 2 到 10。两个阶段之间共有 96 个 lncRNAs 表达显著差异，其中 47 个上调，49 个下调。通路分析发现，靶基因主要集中在 ECM-受体相互作用、Jak-STAT 信号通路、PI3K-Akt 信号通路和 TGF-β 信号通路。本研究揭示了荷斯坦奶牛非泌乳期和早期泌乳期乳腺组织中 lncRNAs 的表达谱和特征，为研究 lncRNAs 在荷斯坦奶牛不同泌乳期的功能提供了基础。
