Barone F C, Clark R K, Feuerstein G, Lenkinski R E, Sarkar S K
Department of Pharmacology, SmithKline Beecham Pharmaceuticals, plc, King of Prussia, PA 19406.
Brain Res Bull. 1991 Feb;26(2):285-91. doi: 10.1016/0361-9230(91)90240-k.
Hemispheric swelling and area of infarction, two parameters of cerebral focal ischemic damage, were identified and quantified from T2-weighted magnetic resonance imaging (MRI) two days after occlusion of the middle cerebral artery (MCAO) in spontaneously hypertensive rat (SHR) brains. Results were compared with these measures quantified from 2,3,5-triphenyltetrazolium hydrochloride (TTC)- and hematoxylin and eosin (H&E)-stained histologic sections in the same brains. The degree of hemispheric swelling and infarct size determined by MRI were highly correlated to the measurements as determined in the TTC- and H&E-stained tissues. These results demonstrate that the focal ischemic damaged area and associated tissue swelling identified by MRI is quantitatively similar to, and thus, is representative of actual tissue damage/changes that can be identified by gross or histologic examination.
在自发性高血压大鼠（SHR）大脑中大脑中动脉闭塞（MCAO）两天后，从T2加权磁共振成像（MRI）中识别并量化了半球肿胀和梗死面积这两个脑局灶性缺血损伤参数。将结果与同一大脑中用2,3,5-三苯基四氮唑盐酸盐（TTC）和苏木精-伊红（H&E）染色的组织学切片所量化的这些测量值进行比较。MRI测定的半球肿胀程度和梗死大小与TTC和H&E染色组织中的测量值高度相关。这些结果表明，MRI识别的局灶性缺血损伤区域和相关组织肿胀在数量上与通过大体或组织学检查所能识别的实际组织损伤/变化相似，因此具有代表性。
