USDA-ARS Plant Science Research Unit, 411 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, MN 55108, USA.
Genome. 2010 Jan;53(1):1-13. doi: 10.1139/g09-079.
The grass species Brachypodium distachyon (hereafter, Brachypodium) has been adopted as a model system for grasses. Here, we describe the development of a genetic linkage map of Brachypodium. The genetic linkage map was developed with an F2 population from a cross between the diploid Brachypodium lines Bd3-1 and Bd21. The map was populated with polymorphic simple sequence repeat (SSR) markers from Brachypodium expressed sequence tag (EST) and bacterial artificial chromosome (BAC) end sequences and conserved orthologous sequence (COS) markers from other grass species. The map is 1386 cM in length and consists of 139 marker loci distributed across 20 linkage groups. Five of the linkage groups exceed 100 cM in length, with the largest being 231 cM long. Assessment of colinearity between the Brachypodium linkage map and the rice genome sequence revealed significant regions of macrosynteny between the two genomes, as well as rearrangements similar to those reported in other grass comparative structural genomics studies. The Brachypodium genetic linkage map described here will serve as a new tool to pursue a range of molecular genetic analyses and other applications in this new model plant system.
短柄草（Brachypodium distachyon，后文简称短柄草）已被广泛用作禾本科模式植物系统。本文描述了短柄草遗传连锁图谱的构建。该图谱以 Bd3-1 和 Bd21 两个二倍体短柄草品系杂交得到的 F2 群体为材料，利用短柄草表达序列标签（EST）和细菌人工染色体（BAC）末端序列中的多态性简单重复序列（SSR）标记以及其他禾本科植物的保守直系同源序列（COS）标记进行构建。图谱全长 1386cM，包含 139 个标记位点，分布在 20 个连锁群上。其中 5 个连锁群的长度超过 100cM，最长的达到 231cM。对短柄草连锁图谱与水稻基因组序列的共线性评估表明，这两个基因组之间存在显著的大片段共线性区域，以及与其他禾本科比较结构基因组学研究中报道的相似的重排。本文构建的短柄草遗传连锁图谱将作为新的工具，用于该新型模式植物系统的一系列分子遗传分析和其他应用。
