利用荧光原位杂交技术鉴定新的冬小麦-冬大麦添加系（6HS 和 7H）和整个 'Martonvásári 9 kr1' - 'Igri' 添加系的稳定性。

Identification of new winter wheat - winter barley addition lines (6HS and 7H) using fluorescence in situ hybridization and the stability of the whole 'Martonvásári 9 kr1' - 'Igri' addition set.

机构信息

Agricultural Research Institute of the Hungarian Academy of Sciences, H-2462 Martonvásár, P.O. Box 19, Hungary.

出版信息

Genome. 2010 Jan;53(1):35-44. doi: 10.1139/g09-085.

PMID:20130747

Abstract

A previous paper reported the development of disomic addition lines (2H, 3H, 4H, and 1HS isochromosomic) from hybrids between the winter wheat 'Martonvásári 9 kr1' and the two-rowed winter barley cultivar 'Igri'. The present paper describes the isolation of two new additions, the 7H disomic and 6HS ditelosomic additions, using fluorescence in situ hybridization with the repetitive DNA probes Afa-family and HvT01. The identification of the barley chromosomes in the wheat genome was confirmed with simple sequence repeat markers. The morphological characterization of the new addition lines is also discussed. Studies of the genetic stability of the whole set (2H, 3H, 4H, 7H, 1HS iso, 6HS) of 'Martonvásári 9 kr1' - 'Igri' additions revealed that the most stable disomic additions are 2H and 3H and the most unstable line is the 1HS isochromosomic addition.

摘要

先前的一篇论文报道了通过冬小麦‘Martonvásári 9 kr1’与二棱冬大麦品种‘Igri’的杂种，发展出了二体附加系（2H、3H、4H 和 1HS 等臂染色体）。本文描述了利用重复 DNA 探针 Afa 家族和 HvT01 通过荧光原位杂交，分离出两个新的附加系，即 7H 二体和 6HS 双着丝粒附加系。利用简单重复序列标记证实了大麦染色体在小麦基因组中的存在。还讨论了新附加系的形态特征。对‘Martonvásári 9 kr1’-‘Igri’整套附加系（2H、3H、4H、7H、1HS 等臂、6HS）的遗传稳定性研究表明，最稳定的二体附加系是 2H 和 3H，最不稳定的是 1HS 等臂染色体附加系。