Taub D D, Rogers T J
Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
Cell Immunol. 1991 Apr 15;134(1):216-24. doi: 10.1016/0008-8749(91)90344-b.
Previous studies from this laboratory have shown that staphylococcal enterotoxin B (SEB) has the capacity to nonspecifically induce multiple T suppressor cell populations which are capable of regulating both primary and secondary in vitro antibody responses. Additional studies have revealed that the suppressive activity of these cells is mediated, at least in part, by an I-J-restricted suppressor-inducer factor. Efforts to characterize the specificity of this inducer molecule have demonstrated the presence of multiple antigen-specific suppressor factors within SEB-stimulated supernatants. Antigen-binding molecules present within these SEB-induced factor preparations were isolated and concentrated over antigen-coupled columns. The results have demonstrated that eluates from trinitrophenyl-ovalbumin and trinitrophenyl-keyhole limpet hemocyanin-coupled columns significantly suppress both primary and secondary anti-TNP plaque-forming colony responses, whereas the filtrates from these columns demonstrated little to no inhibitory activity. The filtrates, but not eluates, from these columns exhibited significant suppressive activity for anti-poly(L-Phe,L-Glu)-poly-DL-Ala-poly-L-Lys, anti-azobenzenearsonate, and anti-sheep red blood cell antibody responses. Additional studies using eluates and filtrates from a variety of antigen-coated columns have demonstrated that suppressor factors of multiple antigenic specificities are present within SEB-induced supernatants. These studies suggest that SEB polyclonally activates antigen-specific suppressor cells which produce suppressor factors which specifically regulate the antibody response.
本实验室先前的研究表明，葡萄球菌肠毒素B（SEB）能够非特异性地诱导多种T抑制细胞群体，这些细胞群体能够调节体外初次和二次抗体反应。进一步的研究表明，这些细胞的抑制活性至少部分是由I-J限制的抑制诱导因子介导的。对这种诱导分子特异性的表征研究表明，SEB刺激的上清液中存在多种抗原特异性抑制因子。在抗原偶联柱上分离并浓缩了这些SEB诱导因子制剂中存在的抗原结合分子。结果表明，从三硝基苯-卵清蛋白和三硝基苯-钥孔血蓝蛋白偶联柱上洗脱的物质能显著抑制初次和二次抗TNP噬斑形成集落反应，而这些柱的滤液几乎没有抑制活性。这些柱的滤液而非洗脱物，对抗聚（L-苯丙氨酸，L-谷氨酸）-聚-DL-丙氨酸-聚-L-赖氨酸、抗偶氮苯砷酸盐和抗绵羊红细胞抗体反应表现出显著的抑制活性。使用来自各种抗原包被柱的洗脱物和滤液进行的进一步研究表明，SEB诱导的上清液中存在多种抗原特异性的抑制因子。这些研究表明，SEB多克隆激活抗原特异性抑制细胞，这些细胞产生特异性调节抗体反应的抑制因子。
