Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran.
Biotechnol Lett. 2010 May;32(5):649-54. doi: 10.1007/s10529-010-0208-x. Epub 2010 Feb 4.
Doxorubicin (DOX), a common cancer chemotherapeutics, was conjugated to folate-modified thiolated-polyethylene glycol-functionalized gold nanoparticles. The in vitro, controlled release behavior of DOX-loaded gold nanoparticles was observed using porous dialysis membranes (cut-off = 2 kDa). DOX-loaded gold nanoparticles had higher cytotoxicity for folate-receptor-positive cells (KB cells) compared to folate-receptor-negative cells (A549 cells) which were 48 and 62% viable for 10 microM doxorubicin, respectively. This indicates the potential of these nano-carriers for targeted-delivery. In addition, healthy cell viability was 69% for 10 microM free doxorubicin whereas for the same content of drug in DOX-loaded nanoparticles healthy cell viability increased to 80%.
阿霉素（DOX）是一种常用的癌症化疗药物，已被共轭到叶酸修饰的巯基化聚乙二醇功能化的金纳米粒子上。使用多孔透析膜（截止值= 2 kDa）观察载 DOX 的金纳米粒子的体外控制释放行为。与叶酸受体阴性细胞（A549 细胞）相比，载 DOX 的金纳米粒子对叶酸受体阳性细胞（KB 细胞）具有更高的细胞毒性，对于 10 μM 的阿霉素，分别有 48%和 62%的细胞活力。这表明这些纳米载体具有靶向递送的潜力。此外，10 μM 游离阿霉素对健康细胞的活力为 69%，而对于载 DOX 纳米粒子中相同含量的药物，健康细胞的活力增加到 80%。
