Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.
J Am Chem Soc. 2010 Mar 3;132(8):2769-74. doi: 10.1021/ja909915m.
We report the use of silicon chips with 16 DNA-modified electrodes (DME chips) utilizing DNA-mediated charge transport for multiplexed detection of DNA and DNA-binding protein targets. Four DNA sequences were simultaneously distinguished on a single DME chip with 4-fold redundancy, including one incorporating a single base mismatch. These chips also enabled investigation of the sequence-specific activity of the restriction enzyme Alu1. DME chips supported dense DNA monolayer formation with high reproducibility, as confirmed by statistical comparison to commercially available rod electrodes. The working electrode areas on the chips were reduced to 10 microm in diameter, revealing microelectrode behavior that is beneficial for high sensitivity and rapid kinetic analysis. These results illustrate how DME chips facilitate sensitive and selective detection of DNA and DNA-binding protein targets in a robust and internally standardized multiplexed format.
我们报告了使用带有 16 个 DNA 修饰电极（DME 芯片）的硅芯片，利用 DNA 介导的电荷传递进行 DNA 和 DNA 结合蛋白靶标的多重检测。在单个 DME 芯片上，通过 4 倍冗余，包括一个包含单个碱基错配的序列，同时区分了 4 个 DNA 序列。这些芯片还能够研究限制酶 Alu1 的序列特异性活性。DME 芯片支持高密度 DNA 单层形成，具有高度的可重复性，这通过与市售棒电极的统计比较得到了证实。芯片上的工作电极面积减小到 10 微米直径，显示出有利于高灵敏度和快速动力学分析的微电极行为。这些结果说明了 DME 芯片如何以稳健和内部标准化的多重格式促进 DNA 和 DNA 结合蛋白靶标的敏感和选择性检测。
