番茄（ Lycopersicon esculentum ）种子：新的类黄酮和细胞毒性作用。

Tomato ( Lycopersicon esculentum ) seeds: new flavonols and cytotoxic effect.

机构信息

CEBAS (CSIC) Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, P.O. Box 164, 30100 Campus University Espinardo, Murcia, Spain.

出版信息

J Agric Food Chem. 2010 Mar 10;58(5):2854-61. doi: 10.1021/jf904015f.

DOI:10.1021/jf904015f

PMID:20131841

Abstract

In this study, seeds of Lycopersicon esculentum Mill. were analyzed by HPLC/UV-PAD/MS(n)-ESI. Fourteen flavonoids were identified, including quercetin, kaempferol, and isorhamnetin derivatives, with 13 of them being reported for the first time in tomato seeds. The major identified compounds were quercetin-3-O-sophoroside, kaempferol-3-O-sophoroside, and isorhamnetin-3-O-sophoroside. A significant cell proliferation inhibition (>80%), against rat basophile leukemia (RBL-2H3) cell line, was observed with this extract (IC(50) = 5980 microg/mL). For acetylcholinesterase inhibitory activity, a concentration-dependent effect was verified (IC(20) = 2400 microg/mL). The same behavior was noted regarding antioxidant capacity, evaluated against DPPH (IC(10) = 284 microg/mL), nitric oxide (IC(25) = 396 microg/L), and superoxide radicals (IC(25) = 3 microg/mL).

摘要

本研究采用 HPLC/UV-PAD/MS(n)-ESI 对番茄种子进行分析。共鉴定出 14 种类黄酮，包括槲皮素、山柰酚和异鼠李素衍生物，其中 13 种类黄酮为首次在番茄种子中发现。主要鉴定出的化合物为槲皮素-3-O-槐糖苷、山柰酚-3-O-槐糖苷和异鼠李素-3-O-槐糖苷。该提取物对大鼠嗜碱性白血病（RBL-2H3）细胞系表现出显著的细胞增殖抑制作用（>80%，IC(50) = 5980μg/mL）。对乙酰胆碱酯酶抑制活性进行了浓度依赖性验证（IC(20) = 2400μg/mL）。在 DPPH（IC(10) = 284μg/mL）、一氧化氮（IC(25) = 396μg/L）和超氧自由基（IC(25) = 3μg/mL）的抗氧化能力评估中也观察到了相同的行为。