W M Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, Washington 99164-2920, USA.
Langmuir. 2010 Apr 6;26(7):4958-64. doi: 10.1021/la903617e.
Bovine serum albumin (BSA) protein incorporated with hydroxyapatite (HA) nanoparticles (NPs) were synthesized by an in situ precipitation process. 2 mol % Zn(2+) and Mg(2+) were used as dopants to synthesize Zn(2+)/Mg(2+)-doped HA-BSA NPs. In our study we used BSA as a model protein. The amount of BSA uptake by doped and undoped HA NPs and subsequent release of BSA from NPs were investigated. Zn-doped HA NPs showed the highest amount of BSA uptake, whereas the amount of BSA loaded in undoped HA NPs was the lowest. A two-stage BSA release profile from doped and undoped HA NPs was observed in phosphate buffer solution (PBS) at pH 7.2 +/- 0.2. The initial burst release was due to the desorption of BSA from the HA surface. The later stage of slow release was controlled by the dissolution of BSA incorporated HA NPs. The BSA release rate from Zn-doped HA NPs was found to be the highest, whereas undoped HA NPs released BSA at the slowest rate. Our study showed that the protein release rate from HA NPs can be controlled by the addition of suitable dopants, and doped HA-based NP systems can be used in bone growth factor and drug release study.
牛血清白蛋白（BSA）与羟基磷灰石（HA）纳米粒子（NPs）结合的蛋白质通过原位沉淀法合成。使用 2 mol%的 Zn(2+)和 Mg(2+)作为掺杂剂来合成 Zn(2+)/Mg(2+)-掺杂 HA-BSA NPs。在我们的研究中，我们使用 BSA 作为模型蛋白。研究了掺杂和未掺杂 HA NPs 对 BSA 的摄取量以及随后从 NPs 中释放 BSA 的情况。Zn 掺杂的 HA NPs 显示出最高的 BSA 摄取量，而未掺杂 HA NPs 中负载的 BSA 量最低。在 pH 7.2±0.2 的磷酸盐缓冲溶液（PBS）中观察到掺杂和未掺杂 HA NPs 的 BSA 释放呈现两阶段释放曲线。初始突释是由于 BSA 从 HA 表面解吸。随后的缓慢释放阶段由掺入 HA NPs 的 BSA 的溶解控制。发现 Zn 掺杂的 HA NPs 中 BSA 的释放速率最高，而未掺杂的 HA NPs 则以最慢的速率释放 BSA。我们的研究表明，通过添加合适的掺杂剂可以控制 HA NPs 中蛋白质的释放速率，并且基于掺杂 HA 的 NP 系统可用于骨生长因子和药物释放研究。
