用于检测沙眼衣原体的Kallested Pathfinder酶免疫分析、细胞离心直接荧光抗体法及细胞培养法的比较

Comparison of the Kallested Pathfinder EIA, cytocentrifuged direct fluorescent antibody, and cell culture for the detection of Chlamydia trachomatis.

作者信息

LeBar W, Schubiner H, Jemal C, Herschman B, Criswell K, Curtin N, Sherbeck M

机构信息

Department of Pathology, Providence Hospital, Southfield, Michigan 48037.

出版信息

Diagn Microbiol Infect Dis. 1991 Jan-Feb;14(1):17-20. doi: 10.1016/0732-8893(91)90081-p.

DOI:10.1016/0732-8893(91)90081-p

PMID:2013206

Abstract

A total of 203 duplicate endocervical samples collected from patients at an adolescent health care center were tested for the presence of Chlamydia trachomatis by cell culture, Pathfinder enzyme immunoassay (EIA) (Kallestad) and cytocentrifuged direct fluorescent antibody (DFA). Compared to cell culture, the Pathfinder assay demonstrated a sensitivity and specificity of 85.2% and 100%, whereas the DFA procedure demonstrated to be 92.6% sensitive and 99.4% specific.

摘要

从一家青少年保健中心的患者那里收集了总共203份重复的宫颈管内膜样本，通过细胞培养、Pathfinder酶免疫测定法（EIA）（凯利斯泰德公司）和细胞离心直接荧光抗体法（DFA）检测沙眼衣原体的存在。与细胞培养相比，Pathfinder测定法的灵敏度和特异性分别为85.2%和100%，而DFA检测程序的灵敏度为92.6%，特异性为99.4%。