用于检测沙眼衣原体的宫颈标本采集的细胞刷
Cytobrush in collection of cervical specimens for detection of Chlamydia trachomatis.
作者信息
Moncada J, Schachter J, Shipp M, Bolan G, Wilber J
机构信息
Department of Laboratory Medicine, University of California, San Francisco 94143.
出版信息
J Clin Microbiol. 1989 Aug;27(8):1863-6. doi: 10.1128/jcm.27.8.1863-1866.1989.
Abstract
Methods of collecting endocervical samples for the detection of Chlamydia trachomatis were evaluated. We compared Calgiswab and cytobrush for isolation in cell culture and Dacron swab and cytobrush for direct fluorescent-antibody (DFA) testing for 632 females attending a sexually transmitted disease clinic. An additional specimen for enzyme immunoassay (EIA) was also collected. True-positives were identified as tissue culture positive and/or both DFA and EIA positive. Use of the cytobrush significantly improved the sensitivity of both the culture (69% with swab and 100% with cytobrush) and DFA testing (68% with swab and 85% with cytobrush). The EIA sensitivity was 85%. The specificity of each test was greater than or equal to 98%. The cytobrush appears to be the superior method for the collection of cervical samples from nonpregnant women.
摘要
对用于检测沙眼衣原体的宫颈内样本采集方法进行了评估。我们比较了用于细胞培养分离的Calgiswab拭子和细胞刷，以及用于直接荧光抗体（DFA）检测的涤纶拭子和细胞刷，检测对象为632名到性传播疾病诊所就诊的女性。另外还采集了一份用于酶免疫测定（EIA）的样本。真阳性被定义为组织培养阳性和/或DFA及EIA均为阳性。使用细胞刷显著提高了培养（拭子培养阳性率为69%，细胞刷培养阳性率为100%）和DFA检测（拭子DFA检测阳性率为68%，细胞刷DFA检测阳性率为85%）的灵敏度。EIA的灵敏度为85%。每项检测的特异性均大于或等于98%。对于非孕妇宫颈样本的采集，细胞刷似乎是更优的方法。
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