Yourassowsky E, Van der Linden M P, Crokaert F
Department of Microbiology, Brugmann University Hospital, Brussels, Belgium.
Chemotherapy. 1991;37(1):38-42. doi: 10.1159/000238830.
Phase contrast microscopy, killing curves and turbidimetric growth curves were used in a comparative study of the antibacterial effects of imipenem and meropenem on Haemophilus influenzae. The minimal inhibitory concentrations (MICs) and their ranges of meropenem and imipenem using five beta-lactamase-producing strains of H. influenzae were 0.03 (0.015-0.06) and 0.6 (0.5-1) micrograms/ml, respectively. Imipenem and meropenem induced spheroplast formation in cultures. Killing curves showed a bacteriostatic activity for meropenem and imipenem for MIC values, and a lag of 2 h in killing for MIC x 2 to MIC x 64. For these concentrations the killing rates of the two antibiotics were similar. Turbidimetric growth curves showed a higher early increase in optical density for meropenem. As far as the MIC value of meropenem was 10 times lower than the MIC value of imipenem, we may conclude that meropenem was more active than imipenem on beta-lactamase-producing strains of H. influenzae.
采用相差显微镜、杀菌曲线和比浊生长曲线，对亚胺培南和美罗培南对流感嗜血杆菌的抗菌作用进行了比较研究。使用5株产β-内酰胺酶的流感嗜血杆菌，美罗培南和亚胺培南的最低抑菌浓度（MIC）及其范围分别为0.03（0.015 - 0.06）和0.6（0.5 - 1）微克/毫升。亚胺培南和美罗培南在培养物中诱导形成原生质球。杀菌曲线显示，美罗培南和亚胺培南对MIC值具有抑菌活性，对于MIC×2至MIC×64的浓度，杀菌存在2小时的延迟。对于这些浓度，两种抗生素的杀菌率相似。比浊生长曲线显示，美罗培南使光密度早期升高幅度更大。由于美罗培南的MIC值比亚胺培南低10倍，我们可以得出结论，美罗培南对产β-内酰胺酶的流感嗜血杆菌菌株的活性比亚胺培南更强。
