Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, PR China.
BMB Rep. 2010 Jan;43(1):34-9. doi: 10.5483/bmbrep.2010.43.1.034.
Expression patterns of OsAREB1 revealed that expression of OsAREB1 gene can be induced by ABA, PEG and heat. Yeast one-hybrid assay demonstrated it can bind to ABA-responsive element (ABRE), which was found in most stress-induced genes. Transgenic Arabidopsis over-expressing OsAREB1 had different responses to ABA and glucose compared to wild-type plants, which suggest OsAREB1 might have a crucial role in these two signaling pathways. Further analysis indicate that OsAREB1 have multiple functions in Arabidopsis. First, OsAREB1 transgenic plants had higher resistance to drought and heat, and OsAREB1 up-regulated the ABA/stress related gene such as RD29A and RD29B. Second, it delayed plant flowering time by down-regulating the expression of flowering-related genes, such as FT, SOC1, LFY and AP1. Due to the dates, OsAREB1 may function as a positive regulator in drought/heat stresses response, but a negative regulator in flowering time in Arabidopsis. [BMB reports 2010; 43(1): 34-39].
OsAREB1 的表达模式表明，该基因的表达可以被 ABA、PEG 和热诱导。酵母单杂交分析表明，它可以结合到大多数应激诱导基因中的 ABA 响应元件（ABRE）上。过表达 OsAREB1 的转基因拟南芥对 ABA 和葡萄糖的反应与野生型植物不同，这表明 OsAREB1 可能在这两个信号通路中具有关键作用。进一步的分析表明，OsAREB1 在拟南芥中具有多种功能。首先，OsAREB1 转基因植物对干旱和热胁迫具有更高的抗性，并且 OsAREB1 上调了与 ABA/胁迫相关的基因，如 RD29A 和 RD29B。其次，它通过下调与开花相关的基因，如 FT、SOC1、LFY 和 AP1，延迟了植物的开花时间。由于时间原因，OsAREB1 可能在拟南芥的干旱/热胁迫反应中作为正调节剂，但在开花时间中作为负调节剂。[BMB 报告 2010；43(1): 34-39]。
