通过蒙古草原植物虎尾草转录组学鉴定抗旱胁迫基因并进行功能分析

Identification and functional analyses of drought stress resistance genes by transcriptomics of the Mongolian grassland plant Chloris virgata.

作者信息

Namuunaa Ganbayar, Bujin Baldorj, Yamagami Ayumi, Bolortuya Byambajav, Kawabata Shintaro, Ogawa Hirotaka, Kanatani Asaka, Shimizu Minami, Minami Anzu, Mochida Keiichi, Miyakawa Takuya, Davaapurev Bekh-Ochir, Asami Tadao, Batkhuu Javzan, Nakano Takeshi

机构信息

Laboratory of Plant Chemical Biology, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan.

RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan.

出版信息

BMC Plant Biol. 2025 Jan 11;25(1):44. doi: 10.1186/s12870-025-06046-3.

DOI:10.1186/s12870-025-06046-3

PMID:39794690

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11724609/

Abstract

BACKGROUND

Mongolian grasslands, including the Gobi Desert, have been exposed to drought conditions with few rains. In such harsh environments, plants with highly resistant abilities against drought stress survive over long periods. We hypothesized that these plants could harbor novel and valuable genes for enhancing drought stress resistance.

RESULTS

In this study, we identified Chloris virgata, a Mongolian grassland plant with strong drought resistance. RNA-seq-based transcriptome analysis was performed to uncover genes associated with drought stress resistance in C. virgata. De novo transcriptome assembly revealed 25,469 protein-coding transcripts and 1,219 upregulated genes after 3- and 6-hr drought stress treatments. Analysis by homology search and Gene Ontology (GO) enrichment indicated that abscisic acid (ABA)- and drought stress-related GO terms were enriched. Among the highly induced genes, ten candidate cDNAs were selected and overexpressed in Arabidopsis. When subjected to drought stress, three of these genes conferred strong drought resistance in the transgenic plants. We named these genes Mongolian Grassland plant Drought-stress resistance genes 1, 2, and 3 (MGD1, MGD2, and MGD3). Gene expression analyses in the transformants suggested that MGD1, MGD2, and MGD3 may activate drought stress-related signalling pathways.

CONCLUSION

This study highlighted the drought resistance of C. virgata and identified three novel genes that enhance drought stress resistance.

摘要

背景

包括戈壁沙漠在内的蒙古草原一直面临少雨的干旱状况。在如此恶劣的环境中，具有高度抗旱能力的植物能够长期存活。我们推测这些植物可能含有增强抗旱性的新的有价值基因。

结果

在本研究中，我们鉴定出了一种具有很强抗旱性的蒙古草原植物虎尾草。基于RNA测序进行转录组分析，以揭示虎尾草中与抗旱性相关的基因。从头转录组组装显示，在3小时和6小时干旱胁迫处理后，有25,469个蛋白质编码转录本和1,219个上调基因。通过同源搜索和基因本体（GO）富集分析表明，脱落酸（ABA）和干旱胁迫相关的GO术语得到了富集。在高度诱导的基因中，选择了10个候选cDNA并在拟南芥中过表达。当受到干旱胁迫时，其中3个基因赋予转基因植物很强的抗旱性。我们将这些基因命名为蒙古草原植物抗旱基因1、2和3（MGD1、MGD2和MGD3）。对转化体的基因表达分析表明，MGD1、MGD2和MGD3可能激活干旱胁迫相关的信号通路。