Department of Medicinal Chemistry, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Islamic Republic of Iran.
Food Chem Toxicol. 2010 May;48(5):1137-44. doi: 10.1016/j.fct.2010.01.028. Epub 2010 Feb 2.
In this study we identified the chemical composition, anti-microbial and antioxidant effects of essential oil and methanolic extract of Hymenocrater longiflorus Benth. Totally 87 volatile compounds from the essential oil in H. longiflorus, were identified by gas chromatography-mass spectrometry (GC-MS). These compounds are mainly monoterpene hydrocarbons, sesquiterpene hydrocarbons, oxygenated monoterpenes and oxygenated sesquiterpenoids compounds. The anti-microbial and antifungal activity of plants extracts against several pathogenic microorganisms was studied by disc diffusion and minimum inhibitory concentration procedures. The results revealed that the essential oil and polar sub-fraction are effective mostly against Staphylococcus aureus, Aspergillus niger and Candida albicans. The antioxidant activity was also determined by 1,1'-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging, beta-carotene linoleic acid assay and reducing power. In addition the total phenol of essential oil (54.6+/-1.2), polar sub-fraction (50.0+/-1.4) and non-polar sub-fraction (64.7+/-2.0) were determined.
在这项研究中，我们确定了长叶海罂粟精油和甲醇提取物的化学成分、抗微生物和抗氧化作用。通过气相色谱-质谱联用仪（GC-MS），从长叶海罂粟精油中鉴定出 87 种挥发性化合物。这些化合物主要是单萜烃、倍半萜烃、含氧单萜和含氧倍半萜化合物。通过圆盘扩散和最小抑菌浓度程序研究了植物提取物对几种病原微生物的抗微生物和抗真菌活性。结果表明，精油和极性亚组分主要对金黄色葡萄球菌、黑曲霉和白色念珠菌有效。抗氧化活性也通过 1,1'-二苯基-2-苦基肼（DPPH）自由基清除、β-胡萝卜素亚油酸测定和还原能力来确定。此外，还测定了精油（54.6+/-1.2）、极性亚组分（50.0+/-1.4）和非极性亚组分（64.7+/-2.0）的总酚含量。
