Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
Food Chem Toxicol. 2010 Jun;48(6):1562-7. doi: 10.1016/j.fct.2010.03.025. Epub 2010 Mar 21.
The objective of the study was to characterize the chemical constituents, antioxidant and antibacterial properties of five ecotypes of Zataria multiflora Boiss. essential oil with respect to main phytogeographic grown towns in Iran. Our investigation showed that the plants had essential oil yield ranging from 1.59+/-0.86% to 0.99+/-0.29% v/w. There were qualitative similarities among the oils from different origins, whereas the amounts of some components were varied. From different samples, 34, 34, 32, 29 and 53 various compounds were identified, representing 98.32%, 97.77%, 99.5%, 98.38% and 96.54% of total oils, respectively. The highest oxygenated monoterpens value in this study stood at 72.99%. Moreover, thymol, a phenolic compound of oxygenated monoterpens, was the most abundant component in GC/MS ranging from 27.05% to 64.87%. In the DPPH antioxidant assay, all samples exhibited a remarkable activity with a higher degree of IC(50)=19.7+/-0.7 microg/ml almost similar to BHT (18.1+/-0.4 microg/ml). Escherichia coli O157:H7 was the most resistant and Candida tropicalis was the most vulnerable organisms with MICs of 16 and 0.062 mg/ml, respectively. In conclusion, it seemed that geographical variation might have influenced the oil compounds either quantitatively or qualitatively.
本研究的目的是描述 5 种不同生态型的藏茴香（Zataria multiflora Boiss.）精油的化学成分、抗氧化和抗菌特性，这些生态型分别来自伊朗的主要植物地理生长城镇。我们的研究表明，植物的精油得率范围为 1.59+/-0.86%至 0.99+/-0.29%（v/w）。不同来源的油之间存在定性相似性，而某些成分的含量则有所不同。从不同的样品中，鉴定出 34、34、32、29 和 53 种不同的化合物，分别代表总油量的 98.32%、97.77%、99.5%、98.38%和 96.54%。在本研究中，含氧单萜的最高含量为 72.99%。此外，在 GC/MS 中，含氧单萜的酚类化合物麝香草酚是最丰富的成分，含量范围为 27.05%至 64.87%。在 DPPH 抗氧化测定中，所有样品均表现出显著的活性，IC（50）值为 19.7+/-0.7 μg/ml，几乎与 BHT（18.1+/-0.4 μg/ml）相当。大肠杆菌 O157:H7 是最具抗性的，热带假丝酵母是最易受影响的，其 MIC 分别为 16 和 0.062 mg/ml。总之，地理变异可能会影响油化合物的数量或质量。
