草果精油的细胞毒性、凋亡和抗氧化活性。
Cytotoxic, apoptotic and antioxidant activity of the essential oil of Amomum tsao-ko.
机构信息
State Key Laboratory of Virology, College of Life Sciences, Center of Nanoscience and Nanotechnology, Wuhan University, Wuhan 430072, People's Republic of China.
出版信息
Bioresour Technol. 2010 Jun;101(11):4205-11. doi: 10.1016/j.biortech.2009.12.131. Epub 2010 Feb 4.
The antioxidant activity, cytotoxicity and mechanism of induction of HepG2 cell death of essential oil from Amomum tsao-ko were determined. The essential oil was cytotoxic to HepG2, Hela, Bel-7402, SGC-7901 and PC-3 cell lines. The lowest IC(50) of 31.80+/-1.18microg/ml was measured for HepG2 carcinoma cell lines. The IC(50) for normal human cell lines (HUVEC and HL-7702) was 163.91+/-5.11-272.41+/-0.97microg/ml. Analyses by flow cytometry, Hoechst 33258 staining and agarose gel electrophoresis indicated that the essential oil induced apoptosis. The essential oil has weak antioxidant activity as measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assay, thiobarbituric acid (TBA) test and ferric reducing antioxidant power (FRAP) assay. The data suggest that the essential oil of A. tsao-ko could be a potential medicinal resource.
采用 DPPH 自由基清除法、硫代巴比妥酸（TBA）法和铁还原抗氧化能力（FRAP）法测定了草果精油的抗氧化活性、细胞毒性及诱导 HepG2 细胞死亡的作用机制。结果表明，草果精油对 HepG2、Hela、Bel-7402、SGC-7901 和 PC-3 细胞系均具有细胞毒性，对 HepG2 癌细胞系的最低 IC50 为 31.80+/-1.18μg/ml，对正常人体细胞系（HUVEC 和 HL-7702）的 IC50 为 163.91+/-5.11-272.41+/-0.97μg/ml。流式细胞术、Hoechst 33258 染色和琼脂糖凝胶电泳分析表明，该精油可诱导细胞凋亡。1,1-二苯基-2-苦基肼基（DPPH）自由基清除法、TBA 法和 FRAP 法测定结果表明，草果精油的抗氧化活性较弱。研究表明，草果精油可能是一种有潜力的药用资源。
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