Cui Qi, Wang Li-Tao, Liu Ju-Zhao, Wang Hui-Mei, Guo Na, Gu Cheng-Bo, Fu Yu-Jie
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, PR China; Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, 150040 Harbin, PR China.
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, PR China; Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, 150040 Harbin, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Sep 1;1061-1062:364-371. doi: 10.1016/j.jchromb.2017.08.001. Epub 2017 Aug 3.
A simple, green and efficient extraction method named modified-solvent free microwave extraction (M-SFME) was employed for the extraction of essential oils (EOs) from Amomun tsao-ko. The process of M-SFME was optimized with the prominent preponderance of such higher extraction yield (1.13%) than those of solvent free microwave extraction (SFME, 0.91%) and hydrodistillation (HD, 0.84%) under the optimal parameters. Thirty-four volatile substances representing 95.4% were identified. The IC values of EOs determined by DPPH radical scavenging activity and β-carotene/linoleic acid bleaching assay were 5.27 and 0.63mg/ml. Furthermore, the EOs exhibited moderate to potent broad-spectrum antimicrobial activity against all tested strains including five gram-positive and two gram-negative bacteria (MIC: 2.94-5.86mg/ml). In general, M-SFME is a potential and desirable alternative for the extraction of EOs from aromatic herbs, and the EOs obtained from A. tsao-ko can be explored as a potent natural antimicrobial and antioxidant preservative ingredient in food industry from the technological and economical points of view.
采用一种名为改良无溶剂微波萃取（M-SFME）的简单、绿色且高效的萃取方法从草果中提取挥发油。在最佳参数下，M-SFME工艺得到了优化，其萃取率显著高于无溶剂微波萃取（SFME，0.91%）和水蒸馏法（HD，0.84%），达到了1.13%。共鉴定出34种挥发性物质，占总量的95.4%。通过DPPH自由基清除活性和β-胡萝卜素/亚油酸漂白试验测定的挥发油IC值分别为5.27和0.63mg/ml。此外，该挥发油对所有测试菌株，包括5种革兰氏阳性菌和2种革兰氏阴性菌，均表现出中度至强效的广谱抗菌活性（最低抑菌浓度：2.94 - 5.86mg/ml）。总体而言，从技术和经济角度来看，M-SFME是从芳香草本植物中提取挥发油的一种有潜力且理想的替代方法，草果挥发油有望作为一种强效的天然抗菌和抗氧化防腐剂成分应用于食品工业。
