Department of Chemistry, Federal University of Viçosa, 36570-000 Viçosa, MG, Brazil.
Bioresour Technol. 2010 Jun;101(11):4056-61. doi: 10.1016/j.biortech.2010.01.012. Epub 2010 Feb 4.
The syringyl/guaiacyl ratio was determined for six different Eucalyptus spp. wood clones cultivated in four regions in Brazil. The determinants were made by pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) and the results were compared with those obtained by alkaline nitrobenzene oxidation method. The S/G ratios were obtained considering all the identified lignin derivatives in the pyrograms and also using two groups of markers. The first group of markers consisted of guaiacol, 4-methylguaiacol, 4-vinylguaiacol, trans-isoeugenol, syringol, 4-methylsyringol, 4-vinylsyringol and trans-4-propenylsyringol compounds as markers. The second group included guaiacol, 4-methylguaiacol, 4-vinylguaiacol, vanillin, 4-ethylsyringol, 4-vinylsyringol, syringaldehyde, syringylacetone and trans-4-propenylsyringol. It was observed from the statistical analysis that the values of S/G obtained by Py-GC-MS using the two groups of markers did not differ significantly from those obtained by nitrobenzene oxidation method.
测定了 6 个不同桉树无性系种在巴西 4 个地区种植的木材的愈创木基/紫丁香基比率。采用热解-气相色谱-质谱法（Py-GC-MS）进行了测定，并将结果与碱性硝基苯氧化法的结果进行了比较。考虑到热图谱中鉴定出的所有木质素衍生物，并使用两组标记物，得出了 S/G 比。第一组标记物由愈创木酚、4-甲基愈创木酚、4-乙烯基愈创木酚、反式异丁香酚、丁香酚、4-甲基丁香酚、4-乙烯基丁香酚和反式 4-丙烯基丁香酚化合物组成。第二组包括愈创木酚、4-甲基愈创木酚、4-乙烯基愈创木酚、香草醛、4-乙基丁香酚、4-乙烯基丁香酚、丁香醛、丁香基丙酮和反式 4-丙烯基丁香酚。从统计分析来看，使用两组标记物的 Py-GC-MS 法得到的 S/G 值与硝基苯氧化法得到的 S/G 值没有显著差异。
