Braciak T A, Gauldie J, Fey G H, Northemann W
Department of Molecular Biology, ELIAS Entwicklungslabor, Freiburg, Germany.
FEBS Lett. 1991 Mar 25;280(2):277-80. doi: 10.1016/0014-5793(91)80311-p.
A stable rat macrophage-derived cell line (RMSV1) was established by transformation of primary peritoneal exudate cells with the SV40 virus. The RMSV1 cell line was used as a model to study the regulation of the interleukin-6 (IL6) gene expression in rat macrophages with respect to lipopolysaccharides (LPS), interleukin-1 (IL1) and glucocorticoids. The IL6 mRNA level in RMSV1 cell lines was induced 20-fold within 4 h by LPS, whereas IL1 had no effect. The glucocorticoids were able to inhibit completely the induction of the IL6 mRNA synthesis by LPS, indicating the negative regulation of the IL6 gene expression by glucocorticoids.
通过用SV40病毒转化原代腹膜渗出细胞，建立了稳定的大鼠巨噬细胞衍生细胞系（RMSV1）。RMSV1细胞系被用作模型，以研究大鼠巨噬细胞中白细胞介素-6（IL6）基因表达相对于脂多糖（LPS）、白细胞介素-1（IL1）和糖皮质激素的调控。RMSV1细胞系中的IL6 mRNA水平在4小时内被LPS诱导了20倍，而IL1没有作用。糖皮质激素能够完全抑制LPS对IL6 mRNA合成的诱导，表明糖皮质激素对IL6基因表达具有负调控作用。
