Scales W E, Chensue S W, Kunkel S L
Department of Pathology, University of Michigan Medical School, Ann Arbor 48109.
Pathobiology. 1992;60(5):289-96. doi: 10.1159/000163737.
We report the expression of both interleukin-6 (IL6) messenger RNA and biological activity in complete Freund's adjuvant-elicited peritoneal macrophages (CFA-M phi). IL6 mRNA expression peaked between 4 and 8 h of lipopolysaccharide (LPS) stimulation; biological activity was maximal at approximately 18 h of stimulation. LPS-induced IL6 mRNA was inhibited by treatment with cycloheximide (5 micrograms/ml), implicating the participation of a secondary protein mediator in the induction process, or the dependence upon protein synthesis for receptor ligand interactions. Comparison of CFA-M phi with resident peritoneal macrophages suggests that the elicited cell population makes more IL6 in response to LPS than the resident population on a per cell basis.
我们报道了在完全弗氏佐剂诱导的腹腔巨噬细胞（CFA-M phi）中白细胞介素-6（IL6）信使核糖核酸（mRNA）的表达及生物活性。脂多糖（LPS）刺激4至8小时期间，IL6 mRNA表达达到峰值；生物活性在刺激约18小时时最大。用环己酰亚胺（5微克/毫升）处理可抑制LPS诱导的IL6 mRNA，这表明在诱导过程中有二级蛋白质介质参与，或者受体配体相互作用依赖蛋白质合成。将CFA-M phi与腹腔常驻巨噬细胞进行比较表明，就单个细胞而言，诱导产生的细胞群体对LPS刺激产生的IL6比常驻细胞群体更多。
