Yagci Artay, Murk William, Stronk Jill, Huszar Gabor
Sperm Physiology Laboratory and Department of Obstetrics, Gynecology, and Reproductive Sciences,Yale University School of Medicine, New Haven, Connecticut, USA.
J Androl. 2010 Nov-Dec;31(6):566-72. doi: 10.2164/jandrol.109.008912. Epub 2010 Feb 4.
During human spermiogenesis, the elongated spermatids undergo a plasma membrane remodeling step that facilitates formation of the zona pellucida and hyaluronic acid (HA) binding sites. Various biochemical sperm markers indicated that human sperm bound to HA exhibit attributes similar to that of zona pellucida-bound sperm, including minimal DNA fragmentation, normal shape, and low frequency of chromosomal aneuploidies. In this work, we tested the hypothesis that HA-bound sperm would be enhanced in sperm of high DNA chain integrity and green acridine orange fluorescence (AOF) compared with the original sperm in semen. Sperm DNA integrity in semen and in their respective HA-bound sperm fractions was studied in 50 men tested for fertility. In the semen samples, the proportions of sperm with green AOF (high DNA integrity) and red AOF (DNA breaks) were 54.9% ± 2.0% and 45.0% ± 1.9%, whereas in the HA-bound sperm fraction, the respective proportions were 99% and 1.0%, respectively. The data indeed demonstrated that HA shows a high degree of selectivity for sperm with high DNA integrity. These findings are important from the points of view of human sperm DNA integrity, sperm function, and the potential efficacy of HA-mediated sperm selection for intracytoplasmic sperm injection.
在人类精子发生过程中，细长的精子细胞会经历质膜重塑步骤，这有助于透明带和透明质酸（HA）结合位点的形成。各种生化精子标志物表明，与透明带结合的精子相比，与HA结合的人类精子表现出相似的特性，包括最小程度的DNA片段化、正常形态以及低频率的染色体非整倍体。在这项研究中，我们检验了一个假设，即与原始精液中的精子相比，具有高DNA链完整性和绿色吖啶橙荧光（AOF）的精子与HA结合后会得到增强。对50名接受生育力检测的男性的精液及其各自与HA结合的精子部分中的精子DNA完整性进行了研究。在精液样本中，具有绿色AOF（高DNA完整性）和红色AOF（DNA断裂）的精子比例分别为54.9%±2.0%和45.0%±1.9%，而在与HA结合的精子部分中，相应比例分别为99%和1.0%。数据确实表明，HA对具有高DNA完整性的精子具有高度选择性。从人类精子DNA完整性、精子功能以及HA介导的精子选择用于胞浆内单精子注射的潜在功效的角度来看，这些发现具有重要意义。
