一种针对植物致病真菌玉米黑粉菌的表达载体。

An expression vector for the phytopathogenic fungus, Ustilago maydis.

作者信息

Kinal H, Tao J S, Bruenn J A

机构信息

Department of Biological Sciences, State University of New York, Buffalo 14260.

出版信息

Gene. 1991 Feb 1;98(1):129-34. doi: 10.1016/0378-1119(91)90115-r.

DOI:10.1016/0378-1119(91)90115-r

PMID:2013404

Abstract

We have constructed an expression vector for the phytopathogenic fungus Ustilago maydis. This vector, pUXV, expresses genes located downstream from a U. maydis glyceraldehyde-3-phosphate dehydrogenase promoter. Plasmid pUXV also contains a selective marker gene conferring resistance to the antibiotic hygromycin B and a U. maydis autonomously replicating sequence, UARS, allowing high transformation efficiency. Expression of a cDNA from the toxin-encoding region of the U. maydis virus P6 in pUXV resulted in as much killing activity as from viral particles when evaluated by killer plate assay. Plasmid pUXV preserves essential sequences from pUC12 and is therefore a shuttle vector for U. maydis and Escherichia coli.

摘要

我们构建了一种用于植物致病真菌玉米黑粉菌（Ustilago maydis）的表达载体。该载体pUXV可表达位于玉米黑粉菌甘油醛-3-磷酸脱氢酶启动子下游的基因。质粒pUXV还包含赋予对潮霉素B抗生素抗性的选择标记基因以及一个玉米黑粉菌自主复制序列UARS，可实现高转化效率。当通过杀伤平板试验评估时，在pUXV中表达来自玉米黑粉菌病毒P6毒素编码区的cDNA所产生的杀伤活性与病毒颗粒产生的杀伤活性相当。质粒pUXV保留了来自pUC12的必需序列，因此是一种用于玉米黑粉菌和大肠杆菌的穿梭载体。