Park C M, Berry J O, Bruenn J A
Department of Biological Sciences, State University of New York at Buffalo, 14260, USA.
Plant Mol Biol. 1996 Jan;30(2):359-66. doi: 10.1007/BF00020122.
Ustilago maydis killer toxins are small polypeptides (7-14 kDa) which kill susceptible cells of closely related fungal species. The KP4 toxin is a single polypeptide subunit with a molecular weight of 11.1 kDa. In this work, a transgenic tobacco plant was constructed which secretes the KP4 toxin at a high level. The KP4 toxin expressed in this transgenic plant was of the same size and specificity as the authentic Ustilago KP4 toxin. The expression level was at least 500 times higher than that of the KP6 toxin expressed in plants. Transgenic crop plants producing the KP4 toxin could be rendered resistant to KP4-susceptible fungal pathogens.
玉米黑粉菌杀伤毒素是小分子量多肽（7 - 14 kDa），可杀死密切相关真菌物种的敏感细胞。KP4毒素是分子量为11.1 kDa的单一多肽亚基。在本研究中，构建了一种高水平分泌KP4毒素的转基因烟草植株。该转基因植株中表达的KP4毒素与天然玉米黑粉菌KP4毒素大小和特异性相同。其表达水平比植物中表达的KP6毒素至少高500倍。产生KP4毒素的转基因作物植株可对KP4敏感型真菌病原体产生抗性。
