Gold S E, Bakkeren G, Davies J E, Kronstad J W
Biotechnology Laboratory, University of British Columbia, Vancouver, Canada.
Gene. 1994 May 16;142(2):225-30. doi: 10.1016/0378-1119(94)90265-8.
Although Ustilago maydis is readily amenable to molecular genetic experimentation, few antibiotic-resistance markers are available for DNA-mediated transformation. This poses constraints on experiments involving targeted gene disruption and complementation. To address this problem, we constructed vectors using one of three additional genes as dominant selectable markers for transformation. Two genes, sat-1 (encoding streptothricin acetyltransferase) and Sh-ble (encoding a phleomycin-resistance polypeptide), are of bacterial origin and have been engineered for expression in Ustilago sp. The third gene encodes an allele of U. maydis beta-tubulin that confers resistance to the fungicide benomyl.
尽管玉米黑粉菌易于进行分子遗传学实验，但用于DNA介导转化的抗生素抗性标记却很少。这对涉及靶向基因破坏和互补的实验造成了限制。为了解决这个问题，我们构建了载体，使用另外三个基因之一作为转化的显性选择标记。两个基因，sat-1（编码链丝菌素乙酰转移酶）和Sh-ble（编码博来霉素抗性多肽），来自细菌，并且经过改造可在玉米黑粉菌中表达。第三个基因编码玉米黑粉菌β-微管蛋白的一个等位基因，该等位基因赋予对杀菌剂苯菌灵的抗性。
